ZFIN Person: Singh, Sumeet Pal

ZFIN ID: ZDB-PERS-090112-4

ZFIN ID: ZDB-PERS-090112-4

Singh, Sumeet Pal

Email:	sumeet.pal.singh@ulb.be
URL:	http://sumeetpalsingh.github.io/
Affiliation:	Sumeet Singh Lab
Address:	IRIBHM ULB Building C, Floor 6, Room C6.126 808 route de Lennik 1070 Brussels
Country:	Belgium
Phone:	+32-2555-4186
Fax:
ORCID ID:	0000-0002-5154-3318

BIOGRAPHY AND RESEARCH INTERESTS

Sumeet Pal Singh, PhD is an alumni of Indian Institute of Technology (IIT), Kanpur. He graduated in 2004 with a B.Tech. degree in Biological Sciences and Bioengineering. Directly after bachelor’s degree, he joined the PhD program at Duke University, USA where he performed his doctorate under the supervision of Dr. Kenneth Poss. For his thesis, Sumeet worked on the cellular basis of bone regeneration in the zebrafish fin amputation model.

For post-doctoral work, Sumeet started work on planarian model system, a fascinating organism with extraordinary regenerative capacity, in the lab of Dr. Jochen Rink at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Germany.

For his second post-doc, he returned to zebrafish research in the lab of Dr. Nikolay Ninov at Center for Regenerative Therapies Dresden (CRTD), Germany. Focussing on the pancreatic beta-cells, Sumeet uncovered latent heterogeneity in the beta-cell population. For this, he developed several transgenic reagents to interrogate the proliferative and functional differences among individual cells. He was awarded an EFSD/Lilly Young Investigator Fellowship for his efforts.

In 2019, Sumeet was awarded the Mandat d’impulsion scientifique - mobilité Ulysse (MISU) by Le Fonds de la Recherche Scientifique (FNRS) to start his group at IRIBHM, part of ULB in Brussels, Belgium.

PUBLICATIONS

Valiente-Gabioud, A.A., Garteizgogeascoa Suñer, I., Idziak, A., Fabritius, A., Basquin, J., Angibaud, J., Nägerl, U.V., Singh, S.P., Griesbeck, O. (2023) Fluorescent sensors for imaging of interstitial calcium. Nature communications. 14:62206220

Pozo-Morales, M., Garteizgogeascoa, I., Perazzolo, C., So, J., Shin, D., Singh, S.P. (2022) In vivo imaging of calcium dynamics in zebrafish hepatocytes. Hepatology (Baltimore, Md.). 77(3):789-801

Singh, S.P., Chawla, P., Hnatiuk, A., Kamel, M., Silva, L.D., Spanjaard, B., Eski, S.E., Janjuha, S., Olivares-Chauvet, P., Kayisoglu, O., Rost, F., Bläsche, J., Kränkel, A., Petzold, A., Kurth, T., Reinhardt, S., Junker, J.P., Ninov, N. (2022) A single-cell atlas of de novo β-cell regeneration reveals the contribution of hybrid β/δ-cells to diabetes recovery in zebrafish. Development (Cambridge, England). 149(2)

Pronobis, M.I., Zheng, S., Singh, S.P., Goldman, J.A., Poss, K.D. (2021) In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. eLIFE. 10:

Gillotay, P., Shankar, M., Haerlingen, B., Sema Elif, E., Pozo-Morales, M., Garteizgogeascoa, I., Reinhardt, S., Kränkel, A., Bläsche, J., Petzold, A., Ninov, N., Kesavan, G., Lange, C., Brand, M., Lefort, A., Libert, F., Detours, V., Costagliola, S., Singh, S.P. (2020) Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland. EMBO reports. 21(12):e50612

Eski, S.E., Dubois, C., Singh, S.P. (2020) Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method. Journal of visualized experiments : JoVE. (160):

Singh, S.P., Ninov, N. (2020) Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2128:159-179

Salem, V., Silva, L.D., Suba, K., Georgiadou, E., Neda Mousavy Gharavy, S., Akhtar, N., Martin-Alonso, A., Gaboriau, D.C.A., Rothery, S.M., Stylianides, T., Carrat, G., Pullen, T.J., Singh, S.P., Hodson, D.J., Leclerc, I., Shapiro, A.M.J., Marchetti, P., Briant, L.J.B., Distaso, W., Ninov, N., Rutter, G.A. (2019) Leader β-cells coordinate Ca²⁺ dynamics across pancreatic islets in vivo. Nature metabolism. 1:615-629

Cox, B.D., De Simone, A., Tornini, V.A., Singh, S.P., Di Talia, S., Poss, K.D. (2018) In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone. Current biology : CB. 28(24):3937-3947.e4

Singh, S.P., Janjuha, S., Chaudhuri, S., Reinhardt, S., Kränkel, A., Dietz, S., Eugster, A., Bilgin, H., Korkmaz, S., Zararsız, G., Ninov, N., Reid, J.E. (2018) Machine learning based classification of cells into chronological stages using single-cell transcriptomics. Scientific Reports. 8:17156

Janjuha, S., Pal Singh, S., Ninov, N. (2018) Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets. Journal of visualized experiments : JoVE. (137)

Janjuha, S., Singh, S.P., Tsakmaki, A., Mousavy-Gharavy, N., Murawala, P., Konantz, J., Birke, S., Hodson, D.J., Rutter, G., Bewick, G., Ninov, N.N. (2018) Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish. eLIFE. 7

Singh, S.P., Janjuha, S., Hartmann, T., Kayisoglu, Ö., Konantz, J., Birke, S., Murawala, P., Alfar, E.A., Murata, K., Eugster, A., Tsuji, N., Morrissey, E.R., Brand, M., Ninov, N. (2017) Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Nature communications. 8:664

Singh, S.P., Holdway, J.E., and Poss, K.D. (2012) Regeneration of amputated zebrafish fin rays from de novo osteoblasts. Developmental Cell. 22(4):879-886

Wang, J., Panáková, D., Kikuchi, K., Holdway, J.E., Gemberling, M., Burris, J.S., Singh, S.P., Dickson, A.L., Lin, Y.F., Sabeh, M.K., Werdich, A.A., Yelon, D., MacRae, C.A., and Poss, K.D. (2011) The regenerative capacity of zebrafish reverses cardiac failure caused by genetic cardiomyocyte depletion. Development (Cambridge, England). 138(16):3421-30

NON-ZEBRAFISH PUBLICATIONS