ZFIN ID: ZDB-LAB-981209-2
Orban Lab
PI/Director: Orban, Laszlo
Contact Person: Orban, Laszlo
Email: laszlo@tll.org.sg
URL: http://www.tll.org.sg/laszlo.asp
Address: Reproductive Genomics Group Temasek Life Sciences Laboratory The National University of Singapore 1 Research Link Singapore, 117 604 Singapore
Country: Singapore
Phone: 65-6872-7413
Fax: 65-6872-7007
Line Designation: sg


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 3 genomic features


STATEMENT OF RESEARCH INTERESTS
•Genetic regulation of gonad development and early gonad differentiation in fish.
•Molecular basis of genetic and environmental sex determination in fish.


LAB MEMBERS
Chang, Alex (Kuok Weai) Graduate Student Liew, Woei Chang Graduate Student Sorowar Hossein, Mohammad Graduate Student
Barabitskaya, Oxana Research Staff Kwan, Xiao Yuen Research Staff Saju, Jolly M. Research Staff
Sreenivasan, Rajini Research Staff Lim, Zijie Lin, Qifeng
May, Natascha


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Lin, Q., Low, L.W.L., Lau, A., Chua, E.W.L., Matsuoka, Y., Lian, Y., Monteiro, A., Tate, S., Gunaratne, J., Carney, T.J. (2019) Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry. Scientific Reports. 9:15240
Wang, Q., Luo, M., Wei, N., Chang, A., Luo, K.Q. (2019) Development of a Liposomal Formulation of Acetyltanshinone IIA for Breast Cancer Therapy. Molecular pharmaceutics. 16(9):3873-3886
Saju, J.M., Hossain, M.S., Liew, W.C., Pradhan, A., Thevasagayam, N.M., Tan, L.S.E., Anand, A., Olsson, P.E., Orbán, L. (2018) Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish. Cell Reports. 25:3252-3261.e4
Wang, Q., Wei, N., Liu, X., Chang, A., Qian Luo, K. (2017) Enhancement of the bioavailability of a novel anticancer compound (acetyltanshinone IIA) by encapsulation within mPEG-PLGA nanoparticles: a study of formulation optimization, toxicity, and pharmacokinetics. Oncotarget. 8(7):12013-12030
Ribas, L., Liew, W.C., Díaz, N., Sreenivasan, R., Orbán, L., Piferrer, F. (2017) Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes. Proceedings of the National Academy of Sciences of the United States of America. 114(6):E941-E950
Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L. (2015) Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation. Stem Cell Reports. 5:156
Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E., Orbán, L. (2015) Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation. Stem Cell Reports. 4(1):61-73
Shen, X.Y., Kwan, H.Y., Thevasagayam, N.M., Prakki, S.R., Kuznetsova, I.S., Ngoh, S.Y., Lim, Z., Feng, F., Chang, A., and Orbán, L. (2014) The first transcriptome and genetic linkage map for Asian arowana. Molecular Ecology Resources. 14(3):622-635
Liew, W.C., and Orbán, L. (2014) Zebrafish sex: a complicated affair. Briefings in Functional Genomics. 13(2):172-87
Sreenivasan, R., Jiang, J., Wang, X., Bartfai, R., Kwan, H.Y., Christoffels, A., and Orban, L. (2014) Gonad Differentiation in Zebrafish Is Regulated by the Canonical Wnt Signaling Pathway. Biology of reproduction. 90(2):45
Pradhan, A., Khalaf, H., Ochsner, S.A., Sreenivasan, R., Koskinen, J., Karlsson, M., Karlsson, J., McKenna, N.J., Orban, L., and Olsson, P.E. (2012) Activation of NF-kappaB prevents the transition from juvenile ovary to testis and promotes ovarian development in zebrafish. The Journal of biological chemistry. 287(45):37926-37938
Liew, W.C., Bartfai, R., Lim, Z., Sreenivasan, R., Siegfried, K.R., and Orban, L. (2012) Polygenic sex determination system in zebrafish. PLoS One. 7(4):e34397
Gilligan, P.C., Kumari, P., Lim, S., Cheong, A., Chang, A., and Sampath, K. (2011) Conservation defines functional motifs in the squint/nodal-related 1 RNA dorsal localization element. Nucleic acids research. 39(8):3340-9
Orban, L., Sreenivasan, R., and Olsson, P.E. (2009) Long and winding roads: Testis differentiation in zebrafish. Molecular and Cellular Endocrinology. 312(1-2):35-41
Sreenivasan, R., Cai, M., Bartfai, R., Wang, X., Christoffels, A., and Orban, L. (2008) Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain. PLoS One. 3(3):e1791
Hossain, M.S., Larsson, A., Scherbak, N., Olsson, P.E., and Orban, L. (2008) Zebrafish Androgen Receptor: Isolation, Molecular, and Biochemical Characterization. Biology of reproduction. 78(2):361-369
Wang, X.G., and Orban, L. (2007) Anti-Mullerian hormone and 11 beta-hydroxylase show reciprocal expression to that of aromatase in the transforming gonad of zebrafish males. Developmental dynamics : an official publication of the American Association of Anatomists. 236(5):1329-1338
Wang, X.G., Bartfai, R., Sleptsova-Freidrich, I., and Orban, L. (2007) The timing and extent of 'juvenile ovary' phase are highly variable during zebrafish testis differentiation. Journal of Fish Biology. 70(sa):33
Christoffels, A., Bartfai, R., Srinivasan, H., Komen, H., and Orban, L. (2006) Comparative genomics in cyprinids: common carp ESTs help the annotation of the zebrafish genome. BMC Bioinformatics. 7 (Suppl 5):S2
Yue, G.H., and Orban, L. (2005) A simple and affordable method for high-throughput DNA extraction from animal tissues for polymerase chain reaction. Electrophoresis. 26(16):3081-3083
Bartfai, R., Balduf, C., Hilton, T., Rathmann, Y., Hadzhiev, Y., Tora, L., Orbán, L., and Muller, F. (2004) TBP2, a Vertebrate-Specific Member of the TBP Family, Is Required in Embryonic Development of Zebrafish. Current biology : CB. 14(7):593-598
Li, Y., Chia, J.M., Bartfai, R., Christoffels, A., Ding, K., Yue, G.H., Ho,M.Y., Hill, J.A., Stupka, E. and Orban, L (2004) Comparative analysis of the testis and ovary transcriptomes in zebrafish by combining experimental and computational tools. Comparative and functional genomics. 5(5):403-418
Bartfai, R. and Orbán, L. (2003) The vasa locus in zebrafish: multiple RGG boxes from intragenic duplications. DNA and cell biology. 22(1):47-54
Adam, A., Bartfai, R., Lele, Z., Krone, P.H., and Orbán, L. (2000) Heat-inducible expression of a reporter gene detected by transient assay in zebrafish. Experimental cell research. 256(1):282-290
Müller, F., Williams, D.W., Kobolak, J., Gauvry, L., Goldspink, G., Orbán, L., and Maclean, N. (1997) Activator effect of coinjected enhancers on the muscle-specific expression of promoters in zebrafish embryos. Molecular reproduction and development. 47(4):404-412
Williams, D. Müller, F., Lavender, F.L., Orbán, L., and Maclean, N. (1996) High transgene activity in the yolk syncytial layer affects quantitative transient expression assays in zebrafish (Danio rerio) embryos. Transgenic Research. 5:433-442
Müller, F., Gauvry, L., Williams, D., Kobolak, J., Maclean, N., Orbán, L., and Goldspink, G. (1996) The use of transient lacZ expression in fish embryos for comparative analysis of activity of cloned regulatory elements. In Gene expression and manipulation in aquatic organisms. S.J. Ennion and G. Goldspink, eds., Soc. Exp. Biol. Sem. Ser. 58, Cambridge, UK: Cambridge University Press. :175-199
Horvath, L. and Orbán, L. (1995) Genome and gene manipulation in the common carp. Aquaculture (Amsterdam, Netherlands). 129:157-181
Szelei, J., Varadi, L., Müller, F., Erdelyi, F., Orbán, L., Horvath, L, and Duda, E. (1994) Liposome-mediated gene transfer in fish embryos. Transgenic Research. 3:116-119
Müller, F., Lele, Z., Varadi, L., Menczel, L., and Orbán, L. (1993) Efficient transient expression system based on square pulse electroporation and in vivo luciferase assay of fertilized fish eggs. FEBS letters. 324:27-32
Müller, F., Ivics, Z., Erdelyi, F. Papp, T., Varadi, L., Horvath, L., Maclean, N., and Orbán, L. (1992) Introducing foreign genes into fish eggs with electroporated sperm as a carrier. Molecular marine biology and biotechnology. 1:276-281