ZFIN ID: ZDB-LAB-170322-1
Johnston Lab
PI/Director: Johnston, Simon
Contact Person: Drew, David
Email: d.drew@sheffield.ac.uk
URL: http://www.johnstonlabs.co.uk
Address: Department of Infection, Immunity and Cardiovascular Disease, School of Medicine Bateson Centre and The Krebs Institute, University of Sheffield
Country: United Kingdom
Phone: +44 (0)114 2222 301
Fax: +44 (0)114 2222 301
Line Designation: sh

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Our lab studies macrophage biology and host pathogen interactions. We are interested in normal immunity to infection and opportunistic infection of the immunocompromised. We combine in vitro immune cell and in vivo zebrafish experimental models with multidisciplinary work with engineers, physicists and mathematicians.

Evans, Robert Post-Doc Fehri, Hamid Graduate Student Gibson, Josie Graduate Student
Kamuyango, Alfred Graduate Student Pline, Kate Graduate Student Bojarczuk, Aleks Research Staff
Drew, David Technical Staff

Sutton, J.A.F., Carnell, O.T., Lafage, L., Gray, J., Biboy, J., Gibson, J.F., Pollitt, E.J.G., Tazoll, S.C., Turnbull, W., Hajdamowicz, N.H., Salamaga, B., Pidwill, G.R., Condliffe, A.M., Renshaw, S.A., Vollmer, W., Foster, S.J. (2021) Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLoS pathogens. 17:e1009468
Gibson, J.F., Prajsnar, T.K., Hill, C.J., Tooke, A.K., Serba, J.J., Tonge, R.D., Foster, S.J., Grierson, A.J., Ingham, P.W., Renshaw, S.A., Johnston, S.A. (2020) Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish. Autophagy. 17(6):1448-1457
Prajsnar, T.K., Serba, J.J., Dekker, B.M., Gibson, J.F., Masud, S., Fleming, A., Johnston, S.A., Renshaw, S.A., Meijer, A.H. (2020) The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils. Autophagy. 17(4):888-902
Evans, R.J., Pline, K., Loynes, C.A., Needs, S., Aldrovandi, M., Tiefenbach, J., Bielska, E., Rubino, R.E., Nicol, C.J., May, R.C., Krause, H.M., O'Donnell, V.B., Renshaw, S.A., Johnston, S.A. (2019) 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLoS pathogens. 15:e1007597
Duvenage, L., Walker, L.A., Bojarczuk, A., Johnston, S.A., MacCallum, D.M., Munro, C.A., Gourlay, C.W. (2019) Inhibition of Classical and Alternative Modes of Respiration in Candida albicans Leads to Cell Wall Remodeling and Increased Macrophage Recognition. mBio. 10(1):
van Leeuwen, L.M., Evans, R.J., Jim, K.K., Verboom, T., Fang, X., Bojarczuk, A., Malicki, J., Johnston, S.A., van der Sar, A.M. (2018) A transgenic zebrafish model for the in vivo study of the blood and choroid plexus brain barriers using claudin 5.. Biology Open. 7(2)
Gilbert, A.S., Seoane, P.I., Sephton-Clark, P., Bojarczuk, A., Hotham, R., Giurisato, E., Sarhan, A.R., Hillen, A., Velde, G.V., Gray, N.S., Alessi, D.R., Cunningham, D.L., Tournier, C., Johnston, S.A., May, R.C. (2017) Vomocytosis of live pathogens from macrophages is regulated by the atypical MAP kinase ERK5. Science advances. 3:e1700898
Dee, C.T., Nagaraju, R.T., Athanasiadis, E.I., Gray, C., Fernandez Del Ama, L., Johnston, S.A., Secombes, C.J., Cvejic, A., Hurlstone, A.F. (2016) CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell-like Populations and Diverse Mononuclear Phagocytes. Journal of immunology (Baltimore, Md. : 1950). 197(9):3520-3530
Bojarczuk, A., Miller, K.A., Hotham, R., Lewis, A., Ogryzko, N.V., Kamuyango, A.A., Frost, H., Gibson, R.H., Stillman, E., May, R.C., Renshaw, S.A., Johnston, S.A. (2016) Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection. Scientific Reports. 6:21489
Robertson, A.L., Holmes, G.R., Bojarczuk, A.N., Burgon, J., Loynes, C.A., Chimen, M., Sawtell, A.K., Hamza, B., Willson, J., Walmsley, S.R., Anderson, S.R., Coles, M.C., Farrow, S.N., Solari, R., Jones, S., Prince, L.R., Irimia, D., Rainger, G.E., Kadirkamanathan, V., Whyte, M.K., Renshaw, S.A. (2014) A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism. Science Translational Medicine. 6:225ra29