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General Information
ZFIN ID: ZDB-LAB-150313-1
Ohshima Lab
PI/Director: Ohshima, Toshio
Contact Person: Shimizu, Yuki
Email: y2llcgipscys@akane.waseda.jp
URL: http://www.ohshima.biomed.sci.waseda.ac.jp/en-index.html
Address: 2-2 Wakamatu-cho, Shinjuku-ku, Tokyo 169-8555, Japan
Country: Japan
Phone: 81-03-5369-7321
Fax: 81-03-5369-7302
Line Designation: wa

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In mammalian, newly generated neurons in the adult brain plays an important role for learning and memory. In addition, in the medical aspect, regulation of the proliferation and differentiation of the neural stem cells (NSCs) is one of the key technique for regenerative medicine of central nervous system (CNS). NSCs transplantation strategies might have therapeutic promise in treating adult CNS disorders characterized by loss of neuronal or glial cells. In the adult teleost brain, NSCs are distributed in various brain regions, and continue to produce new neurons and glial cells through their life. We focus on the molecular mechanisms of proliferation and differentiation of NSCs in the adult zebrafish optic tectum, and try to reveal the general molecular machinery of proliferation and differentiation of NSCs.

Tanaka, Hideomi Post-Doc Ito, Ryoichi Graduate Student Kobayashi, Shunsuke Graduate Student
Sato, Yuki Graduate Student Akashi, Hayato Shimizu, Yuki
Ueda, Yuto

Ueda, Y., Shimizu, Y., Shimizu, N., Ishitani, T., Ohshima, T. (2018) Involvement of Sonic hedgehog and Notch signaling in regenerative neurogenesis in adult zebrafish optic tectum after stab injury. The Journal of comparative neurology. 526(15):2360-2372
Shimizu, Y., Ueda, Y., Ohshima, T. (2018) Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish. Glia. 66(7):1382-1394
Shitasako, S., Ito, Y., Ito, R., Ueda, Y., Shimizu, Y., Ohshima, T. (2017) Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish. Developmental Neurobiology. 77(10):1206-1220
Nozawa, K., Lin, Y., Kubodera, R., Shimizu, Y., Tanaka, H., Ohshima, T. (2017) Zebrafish Mecp2 is required for proper axonal elongation of motor neurons and synapse formation. Developmental Neurobiology. 77(9):1101-1113
Sato, Y., Yano, H., Shimizu, Y., Tanaka, H., Ohshima, T. (2017) Optic nerve input-dependent regulation of neural stem cell proliferation in the optic tectum of adult zebrafish. Developmental Neurobiology. 77(4):474-482
Shimizu, Y., Ito, Y., Tanaka, H., Ohshima, T. (2015) Radial glial cell-specific ablation in the adult zebrafish brain. Genesis (New York, N.Y. : 2000). 53(7):431-9
Dozawa, M., Kono, H., Sato, Y., Ito, Y., Tanaka, H., Ohshima, T. (2014) Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental dynamics : an official publication of the American Association of Anatomists. 243(11):1401-15
Morimura, R., Nozawa, K., Tanaka, H., and Ohshima, T. (2013) Phosphorylation of Dpsyl2 (CRMP2) and Dpsyl3 (CRMP4) is required for positioning of caudal primary motor neurons in the zebrafish spinal cord. Developmental Neurobiology. 73(12):911-20
Brondolin, M., Berger, S., Reinke, M., Tanaka, H., Ohshima, T., Fuss, B., and Hoch, M. (2013) Identification and expression analysis of the zebrafish homologs of the ceramide synthase gene family. Developmental dynamics : an official publication of the American Association of Anatomists. 242(2):189-200
Tanaka, H., Morimura, R., and Ohshima, T. (2012) Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish. Developmental Biology. 370(2):223-236
Ohata, S., Aoki, R., Kinoshita, S., Yamaguchi, M., Tsuruoka-Kinoshita, S., Tanaka, H., Wada, H., Watabe, S., Tsuboi, T., Masai, I., and Okamoto, H. (2011) Dual Roles of Notch in Regulation of Apically Restricted Mitosis and Apicobasal Polarity of Neuroepithelial Cells. Neuron. 69(2):215-230
Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H. (2011) Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. Developmental dynamics : an official publication of the American Association of Anatomists. 240(1):9-22
Ito, Y., Tanaka, H., Okamoto, H., and Ohshima, T. (2010) Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum. Developmental Biology. 342(1):26-38
Ohata, S., Kinoshita, S., Aoki, R., Tanaka, H., Wada, H., Tsuruoka-Kinoshita, S., Tsuboi, T., Watabe, S., and Okamoto, H. (2009) Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain. Development (Cambridge, England). 136(10):1653-1663
Nishiwaki, Y., Komori, A., Sagara, H., Suzuki, E., Manabe, T., Hosoya, T., Nojima, Y., Wada, H., Tanaka, H., Okamoto, H., and Masai, I. (2008) Mutation of cGMP phosphodiesterase 6alpha'-subunit gene causes progressive degeneration of cone photoreceptors in zebrafish. Mechanisms of Development. 125(11-12):932-946
Tanaka, H., Maeda, R., Shoji, W., Wada, H., Masai, I., Shiraki, T., Kobayashi, M., Nakayama, R., and Okamoto, H. (2007) Novel mutations affecting axon guidance in zebrafish and a role for plexin signalling in the guidance of trigeminal and facial nerve axons. Development (Cambridge, England). 134(18):3259-3269
Wada, H., Tanaka, H., Nakayama, S., Iwasaki, M., and Okamoto, H. (2006) Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain. Development (Cambridge, England). 133(23):4749-4759
Wada, H., Iwasaki, M., Sato, T., Masai, I., Nishiwaki, Y., Tanaka, H., Sato, A., Nojima, Y., and Okamoto, H. (2005) Dual roles of zygotic and maternal Scribble1 in neural migration and convergent extension movements in zebrafish embryos. Development (Cambridge, England). 132(10):2273-2285
Kawakami, A., Nojima, Y., Toyoda, A., Takahoko, M., Satoh, M., Tanaka, H., Wada, H., Masai, I., Terasaki, H., Sakaki, Y., Takeda, H., and Okamoto, H. (2005) The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling. Current biology : CB. 15(5):480-488
Masai, I., Lele, Z., Yamaguchi, M., Komori, A., Nakata, A., Nishiwaki, Y., Wada, H., Tanaka, H., Nojima, Y., Hammerschmidt, M., Wilson, S.W., and Okamoto, H. (2003) N-cadherin mediates retinal lamination, maintenance of forebrain compartments and patterning of retinal neurites. Development (Cambridge, England). 130(11):2479-2494
Tanaka, H., Abe, K., and Kim, C.-H. (1997) Cloning and expression of the quaking gene in the zebrafish embryo. Mechanisms of Development. 69:209-213
Kim, C.H., Ueshima, E., Muraoka, O., Tanaka, H., Yeo, S.Y., Huh, T.L., and Miki, N. (1996) Zebrafish elav/huC homologue as a very early neuronal marker. Neuroscience letters. 216(2):109-112
Kuo, C.H., Uetsuki, T., Kim, C.H., Tanaka, H., Li, B.S., Taira, E., Higuchi, H., Okamoto, H., Yoshikawa, K., and Miki, N. (1995) Determination of a necdin cis-acting element required for neuron specific expression by using zebra fish. Biochemical and Biophysical Research Communications. 211(2):438-446