ZFIN ID: ZDB-LAB-150304-2
Kindt Lab
PI/Director: Kindt, Katie
Contact Person: Kindt, Katie
Email: katie.kindt@nih.gov
URL: https://irp.nih.gov/pi/katie-kindt
Address: National Institute on Deafness and Other Communication Disorders Building 35A, Room 1D 908 35A Convent Drive Bethesda, MD 20892 USA
Country: United States
Phone: 301-402-2684
Fax:
Line Designation: idc


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 23 genomic features


STATEMENT OF RESEARCH INTERESTS
Auditory and vestibular systems are required for proper hearing and
balance. These systems require sensory hair cells of the inner ear, to
sense sound and process vestibular stimuli. To reliably transmit auditory
and vestibular information, hair cells use specialized pre-synaptic
structures called synaptic ribbons. Our studies combine genetic,
molecular, and imaging-based approaches to identify the structural and
functional processes underlying synapse formation in hair cells. For our
studies we use the zebrafish lateral-line system in order to study
hair-cell development and function, in a live, transparent preparation. We
have an extremely powerful collection of transgenic zebrafish that label
synaptic structures to either assess synaptic morphology or function using
genetically encoded fluorescent proteins. We are combining these
microscopy-based approaches with CRISPR technology to create mutant
zebrafish in order to identify genes required for synapse formation,
function and regeneration. With this knowledge we aim to apply our
understanding of these processes in order to understand how to properly
reform hair cells and synaptic structures when they are loss or damaged
after hearing loss.


LAB MEMBERS
Cebel, Elizabeth Post-Doc Hussain, Saman Post-Doc Sandeep, David Graduate Student
Lear, Sophie Research Staff Lei, Zhengchang Research Staff Pinter, Katherine Research Staff
Tadesse, Yommi Research Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Kindig, K., Stepanyan, R., Kindt, K.S., McDermott, B.M. (2023) Asymmetric mechanotransduction by hair cells of the zebrafish lateral line. Current biology : CB. 33(7):1295-1307.e3
Angueyra, J.M., Kunze, V.P., Patak, L.K., Kim, H., Kindt, K., Li, W. (2023) Transcription factors underlying photoreceptor diversity. eLIFE. 12:
Saettele, A.L., Wong, H.C., Kindt, K.S., Warchol, M.E., Sheets, L. (2022) Prolonged Dexamethasone Exposure Enhances Zebrafish Lateral-Line Regeneration But Disrupts Mitochondrial Homeostasis and Hair Cell Function. Journal of the Association for Research in Otolaryngology : JARO. 23(6):683-700
Lukasz, D., Beirl, A., Kindt, K. (2022) Chronic neurotransmission increases the susceptibility of lateral-line hair cells to ototoxic insults. eLIFE. 11:
Zhang, Q., Kindt, K.S. (2022) Using Light-Sheet Microscopy to Study Spontaneous Activity in the Developing Lateral-Line System. Frontiers in cell and developmental biology. 10:819612
Kindt, K.S., Akturk, A., Jarysta, A., Day, M., Beirl, A., Flonard, M., Tarchini, B. (2021) EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia. Nature communications. 12:2861
Sheets, L., Holmgren, M., Kindt, K.S. (2021) How Zebrafish Can Drive the Future of Genetic-based Hearing and Balance Research. Journal of the Association for Research in Otolaryngology : JARO. 22(3):215-235
Hudson, A.M., Lockard, G.M., Namjoshi, O.A., Wilson, J.W., Kindt, K.S., Blough, B.E., Coffin, A.B. (2020) Berbamine Analogs Exhibit Differential Protective Effects From Aminoglycoside-Induced Hair Cell Death. Frontiers in Cellular Neuroscience. 14:234
Wong, H.C., Zhang, Q., Beirl, A.J., Petralia, R.S., Wang, Y.X., Kindt, K. (2019) Synaptic mitochondria regulate hair-cell synapse size and function. eLIFE. 8:
Manchanda, A., Chatterjee, P., Bonventre, J.A., Haggard, D.E., Kindt, K.S., Tanguay, R.L., Johnson, C.P. (2019) Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish. Scientific Reports. 9:14273
Lukasz, D., Kindt, K.S. (2018) In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish. Journal of visualized experiments : JoVE. (141):
Angueyra, J.M., Kindt, K.S. (2018) Leveraging Zebrafish to Study Retinal Degenerations. Frontiers in cell and developmental biology. 6:110
Kindt, K.S., Sheets, L. (2018) Transmission Disrupted: Modeling Auditory Synaptopathy in Zebrafish. Frontiers in cell and developmental biology. 6:114
Matern, M.S., Beirl, A., Ogawa, Y., Song, Y., Paladugu, N., Kindt, K.S., Hertzano, R. (2018) Corrigendum: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag. Frontiers in cell and developmental biology. 6:84
Matern, M.S., Beirl, A., Ogawa, Y., Song, Y., Paladugu, N., Kindt, K.S., Hertzano, R. (2018) Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag. Frontiers in cell and developmental biology. 6:47
Ji, Y.R., Warrier, S., Jiang, T., Wu, D.K., Kindt, K. (2018) Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells. eLIFE. 7
Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S. (2018) Synaptically silent sensory hair cells in zebrafish are recruited after damage. Nature communications. 9:1388
Imtiaz, A., Belyantseva, I.A., Beirl, A.J., Fenollar-Ferrer, C., Bashir, R., Bukhari, I., Bouzid, A., Shaukat, U., Azaiez, H., Booth, K.T., Kahrizi, K., Najmabadi, H., Maqsood, A., Wilson, E.A., Fitzgerald, T.S., Tlili, A., Olszewski, R., Lund, M., Chaudhry, T., Rehman, A.U., Starost, M.F., Waryah, A.M., Hoa, M., Dong, L., Morell, R.J., Smith, R.J.H., Riazuddin, S., Masmoudi, S., Kindt, K., Naz, S., Friedman, T.B. (2017) CDC14A Phosphatase is Essential for Hearing and Male Fertility in Mouse and Human. Human molecular genetics. 27(5):780-798
Graydon, C.W., Manor, U., Kindt, K.S. (2017) In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses. Scientific Reports. 7:7467
Sheets, L., He, X.J., Olt, J., Schreck, M., Petralia, R.S., Wang, Y.X., Zhang, Q., Beirl, A., Nicolson, T., Marcotti, W., Trapani, J.G., Kindt, K.S. (2017) Enlargement of ribbons in zebrafish hair cells increases calcium currents, but disrupts afferent spontaneous activity and timing of stimulus onset. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(26):6299-6313
Jiang, T., Kindt, K., Wu, D.K. (2017) Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells. eLIFE. 6
Pei, W., Xu, L., Varshney, G.K., Carrington, B., Bishop, K., Jones, M., Huang, S.C., Idol, J., Pretorius, P.R., Beirl, A., Schimmenti, L.A., Kindt, K.S., Sood, R., Burgess, S.M. (2016) Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases. Scientific Reports. 6:29946
Zhang, Q.X., He, X.J., Wong, H.C., Kindt, K.S. (2016) Functional calcium imaging in zebrafish lateral-line hair cells. Methods in cell biology. 133:229-252
Maeda, R., Kindt, K.S., Mo, W., Morgan, C.P., Erickson, T., Zhao, H., Clemens-Grisham, R., Barr-Gillespie, P.G., Nicolson, T. (2014) Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2. Proceedings of the National Academy of Sciences of the United States of America. 111(35):12907-12
Grisham, R.C., Kindt, K., Finger-Baier, K., Schmid, B., and Nicolson, T. (2013) Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair Cells. PLoS One. 8(4):e60866
Sheets, L., Kindt, K.S., and Nicolson, T. (2012) Presynaptic CaV1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(48):17273-17286
Kindt, K.S., Finch, G., and Nicolson, T. (2012) Kinocilia mediate mechanosensitivity in developing zebrafish hair cells. Developmental Cell. 23(2):329-341