ZFIN Lab: Zebrafish Lab for Nephrology

ZFIN ID: ZDB-LAB-120626-1

Zebrafish Lab for Nephrology

PI/Director:	Zhou, Weibin
Contact Person:	Zhou, Weibin
Email:	weibin.zhou@mssm.edu
URL:
Address:	Division of Nephrology Department of Medicine Icahn School of Medicine at Mount Sinai 1468 Madison Ave. Box 1243 Renal Division Annenberg 23rd Floor Room 23-53 New York, NY 10029
Country:	United States
Phone:	+1 (212)241-2318
Fax:	+1 (212)987-0389
Line Designation:	mss

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 22 genomic features

STATEMENT OF RESEARCH INTERESTS

Renal glomerular diseases, frequently causing glomerulosclerosis and chronic kidney diseases (CKD), are the leading cause of end-stage kidney disease (ESKD). We are particularly interested in using zebrafish as a new model organism for the study of glomerular function and disorders.

LAB MEMBERS

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Nishimura, Y., Ishii, T., Ando, K., Yuge, S., Nakajima, H., Zhou, W., Mochizuki, N., Fukuhara, S. (2022) Blood Flow Regulates Glomerular Capillary Formation in Zebrafish Pronephros. Kidney360. 3:700-713

Siegerist, F., Lange, T., Iervolino, A., Koppe, T.M., Zhou, W., Capasso, G., Endlich, K., Endlich, N. (2021) Evaluation of endogenous miRNA reference genes across different zebrafish strains, developmental stages and kidney disease models. Scientific Reports. 11:22894

Hansen, K.U.I., Siegerist, F., Daniel, S., Schindler, M., Iervolino, A., Blumenthal, A., Daniel, C., Amann, K., Zhou, W., Endlich, K., Endlich, N. (2020) Prolonged podocyte depletion in larval zebrafish resembles mammalian focal and segmental glomerulosclerosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 34(12):15961-15974

Lee, M.S., Philippe, J., Katsanis, N., Zhou, W. (2019) Polyketide Synthase Plays a Conserved Role in Otolith Formation. Zebrafish. 16(4):363-369

Schulz, A., Müller, N.V., van de Lest, N.A., Eisenreich, A., Schmidbauer, M., Barysenka, A., Purfürst, B., Sporbert, A., Lorenzen, T., Meyer, A.M., Herlan, L., Witten, A., Rühle, F., Zhou, W., de Heer, E., Scharpfenecker, M., Panáková, D., Stoll, M., Kreutz, R. (2019) Analysis of the genomic architecture of a complex trait locus in hypertensive rat models links Tmem63c to kidney damage. eLIFE. 8:

Hosono, Y., Niknafs, Y.S., Prensner, J.R., Iyer, M.K., Dhanasekaran, S.M., Mehra, R., Pitchiaya, S., Tien, J., Escara-Wilke, J., Poliakov, A., Chu, S.C., Saleh, S., Sankar, K., Su, F., Guo, S., Qiao, Y., Freier, S.M., Bui, H.H., Cao, X., Malik, R., Johnson, T.M., Beer, D.G., Feng, F.Y., Zhou, W., Chinnaiyan, A.M. (2017) Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171:1559-1572.e20

Xu, D., Zhou, W., Peng, L. (2017) Three-dimensional live multi-label light-sheet imaging with synchronous excitation-multiplexed structured illumination. Optics express. 25:31159-31173

Siegerist, F., Zhou, W., Endlich, K., Endlich, N. (2017) 4D in vivo imaging of glomerular barrier function in a zebrafish podocyte injury model. Acta physiologica (Oxford, England). 220(1):167-173

Siegerist, F., Blumenthal, A., Zhou, W., Endlich, K., Endlich, N. (2017) Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae. Scientific Reports. 7:43655

Xu, D., Zhou, W., Peng, L. (2017) Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam. Biomedical Optics Express. 8:570-578

Wan, X., Lee, M.S., Zhou, W. (2016) Dosage-dependent Role of RAC1 in Podocyte Injury. American journal of physiology. Renal physiology. 310(8):F777-F784

Wan, X., Chen, Z., Choi, W.I., Gee, H.Y., Hildebrandt, F., Zhou, W. (2016) Loss of Epithelial Membrane Protein 2 Aggravates Podocyte Injury via Upregulation of Caveolin-1. Journal of the American Society of Nephrology : JASN. 27(4):1066-75

Chen, Z., Wan, X., Hou, Q., Shi, S., Wang, L., Chen, P., Zhu, X., Zeng, C., Qin, W., Zhou, W., Liu, Z. (2016) GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway. Cell Death & Disease. 7:e2068

Zhao, M., Wan, X., Li, Y., Zhou, W., Peng, L. (2015) Multiplexed 3D FRET imaging in deep tissue of live embryos. Scientific Reports. 5:13991

Gee, H.Y., Zhang, F., Ashraf, S., Kohl, S., Sadowski, C.E., Vega-Warner, V., Zhou, W., Lovric, S., Fang, H., Nettleton, M., Zhu, J.Y., Hoefele, J., Weber, L.T., Podracka, L., Boor, A., Fehrenbach, H., Innis, J.W., Washburn, J., Levy, S., Lifton, R.P., Otto, E.A., Han, Z., Hildebrandt, F. (2015) KANK deficiency leads to podocyte dysfunction and nephrotic syndrome. The Journal of Clinical Investigation. 125(6):2375-84

Lee, M.S., Hwang, K.S., Oh, H.W., Ji-Ae, K., Kim, H.T., Cho, H.S., Lee, J.J., Yeong Ko, J., Choi, J.H., Jeong, Y.M., You, K.H., Kim, J., Park, D.S., Nam, K.H., Aizawa, S., Kiyonari, H., Shioi, G., Park, J.H., Zhou, W., Kim, N.S., Kim, C.H. (2015) IFT46 plays an essential role in cilia development. Developmental Biology. 400(2):248-57

Ogino, K., Low, S.E., Yamada, K., Saint-Amant, L., Zhou, W., Muto, A., Asakawa, K., Nakai, J., Kawakami, K., Kuwada, J.Y., Hirata, H. (2015) RING finger protein 121 facilitates the degradation and membrane localization of voltage-gated sodium channels. Proceedings of the National Academy of Sciences of the United States of America. 112(9):2859-64

Schueler, M., Braun, D.A., Chandrasekar, G., Gee, H.Y., Klasson, T.D., Halbritter, J., Bieder, A., Porath, J.D., Airik, R., Zhou, W., LoTurco, J.J., Che, A., Otto, E.A., Böckenhauer, D., Sebire, N.J., Honzik, T., Harris, P.C., Koon, S.J., Gunay-Aygun, M., Saunier, S., Zerres, K., Bruechle, N.O., Drenth, J.P., Pelletier, L., Tapia-Páez, I., Lifton, R.P., Giles, R.H., Kere, J., Hildebrandt, F. (2015) DCDC2 Mutations Cause a Renal-Hepatic Ciliopathy by Disrupting Wnt Signaling. American journal of human genetics. 96(1):81-92

Huang, L., Xiao, A., Wecker, A., McBride, D.A., Choi, S.Y., Zhou, W., Lipschutz, J.H. (2014) A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys. Journal of visualized experiments : JoVE. (94)

Gee, H.Y., Ashraf, S., Wan, X., Vega-Warner, V., Esteve-Rudd, J., Lovric, S., Fang, H., Hurd, T.W., Sadowski, C.E., Allen, S.J., Otto, E.A., Korkmaz, E., Washburn, J., Levy, S., Williams, D.S., Bakkaloglu, S.A., Zolotnitskaya, A., Ozaltin, F., Zhou, W., Hildebrandt, F. (2014) Mutations in EMP2 Cause Childhood-Onset Nephrotic Syndrome. American journal of human genetics. 94(6):884-90

Zhao, M., Zhang, H., Li, Y., Ashok, A., Liang, R., Zhou, W., Peng, L. (2014) Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy. Biomedical Optics Express. 5:1296-308

Arif, E., Kumari, B., Wagner, M.C., Zhou, W., Holzman, L.B., and Nihalani, D. (2013) Myo1c is an unconventional myosin required for zebrafish glomerular development. Kidney International. 84(6):1154-65

Ashraf, S., Gee, H.Y., Woerner, S., Xie, L.X., Vega-Warner, V., Lovric, S., Fang, H., Song, X., Cattran, D.C., Avila-Casado, C., Paterson, A.D., Nitschké, P., Bole-Feysot, C., Cochat, P., Esteve-Rudd, J., Haberberger, B., Allen, S.J., Zhou, W., Airik, R., Otto, E.A., Barua, M., Al-Hamed, M.H., Kari, J.A., Evans, J., Bierzynska, A., Saleem, M.A., Böckenhauer, D., Kleta, R., El Desoky, S., Hacihamdioglu, D.O., Gok, F., Washburn, J., Wiggins, R.C., Choi, M., Lifton, R.P., Levy, S., Han, Z., Salviati, L., Prokisch, H., Williams, D.S., Pollak, M., Clarke, C.F., Pei, Y., Antignac, C., and Hildebrandt, F. (2013) ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption. J. Clin. Invest.. 123(12):5179-5189

Miller, D.L., and Zhou, W. (2013) A System for Investigation of Biological Effects of Diagnostic Ultrasound on Development of Zebrafish Embryos. Zebrafish. 10(4):459-65

Zariwala, M.A., Gee, H.Y., Kurkowiak, M., Al-Mutairi, D.A., Leigh, M.W., Hurd, T.W., Hjeij, R., Dell, S.D., Chaki, M., Dougherty, G.W., Adan, M., Spear, P.C., Esteve-Rudd, J., Loges, N.T., Rosenfeld, M., Diaz, K.A., Olbrich, H., Wolf, W.E., Sheridan, E., Batten, T.F., Halbritter, J., Porath, J.D., Kohl, S., Lovric, S., Hwang, D.Y., Pittman, J.E., Burns, K.A., Ferkol, T.W., Sagel, S.D., Olivier, K.N., Morgan, L.C., Werner, C., Raidt, J., Pennekamp, P., Sun, Z., Zhou, W., Airik, R., Natarajan, S., Allen, S.J., Amirav, I., Wieczorek, D., Landwehr, K., Nielsen, K., Schwerk, N., Sertic, J., Köhler, G., Washburn, J., Levy, S., Fan, S., Koerner-Rettberg, C., Amselem, S., Williams, D.S., Mitchell, B.J., Drummond, I.A., Otto, E.A., Omran, H., Knowles, M.R., and Hildebrandt, F. (2013) ZMYND10 Is Mutated in Primary Ciliary Dyskinesia and Interacts with LRRC6. American journal of human genetics. 93(2):336-345

Gee, H.Y., Saisawat, P., Ashraf, S., Hurd, T.W., Vega-Warner, V., Fang, H., Beck, B.B., Gribouval, O., Zhou, W., Diaz, K.A., Natarajan, S., Wiggins, R.C., Lovric, S., Chernin, G., Schoeb, D.S., Ovunc, B., Frishberg, Y., Soliman, N.A., Fathy, H.M., Goebel, H., Hoefele, J., Weber, L.T., Innis, J.W., Faul, C., Han, Z., Washburn, J., Antignac, C., Levy, S., Otto, E.A., and Hildebrandt, F. (2013) ARHGDIA mutations cause nephrotic syndrome via defective RHO GTPase signaling. J. Clin. Invest.. 123(8):3243-3253

Hurd, T.W., Otto, E.A., Mishima, E., Gee, H.Y., Inoue, H., Inazu, M., Yamada, H., Halbritter, J., Seki, G., Konishi, M., Zhou, W., Yamane, T., Murakami, S., Caridi, G., Ghiggeri, G., Abe, T., and Hildebrandt, F. (2013) Mutation of the Mg2+ Transporter SLC41A1 Results in a Nephronophthisis-Like Phenotype. Journal of the American Society of Nephrology : JASN. 24(6):967-977

Horstick, E.J., Linsley, J.W., Dowling, J.J., Hauser, M.A., McDonald, K.K., Ashley-Koch, A., Saint-Amant, L., Satish, A., Cui, W.W., Zhou, W., Sprague, S.M., Stamm, D.S., Powell, C.M., Speer, M.C., Franzini-Armstrong, C., Hirata, H., and Kuwada, J.Y. (2013) Stac3 is a component of the excitation-contraction coupling machinery and mutated in Native American myopathy. Nature communications. 4:1952

Chaki, M., Airik, R., Ghosh, A.K., Giles, R.H., Chen, R., Slaats, G.G., Wang, H., Hurd, T.W., Zhou, W., Cluckey, A., Gee, H.Y., Ramaswami, G., Hong, C.J., Hamilton, B.A., Cervenka, I., Ganji, R.S., Bryja, V., Arts, H.H., van Reeuwijk, J., Oud, M.M., Letteboer, S.J., Roepman, R., Husson, H., Ibraghimov-Beskrovnaya, O., Yasunaga, T., Walz, G., Eley, L., Sayer, J.A., Schermer, B., Liebau, M.C., Benzing, T., Le Corre, S., Drummond, I., Janssen, S., Allen, S.J., Natarajan, S., O'Toole, J.F., Attanasio, M., Saunier, S., Antignac, C., Koenekoop, R.K., Ren, H., Lopez, I., Nayir, A., Stoetzel, C., Dollfus, H., Massoudi, R., Gleeson, J.G., Andreoli, S.P., Doherty, D.G., Lindstrad, A., Golzio, C., Katsanis, N., Pape, L., Abboud, E.B., Al-Rajhi, A.A., Lewis, R.A., Omran, H., Lee, E.Y., Wang, S., Sekiguchi, J.M., Saunders, R., Johnson, C.A., Garner, E., Vanselow, K., Andersen, J.S., Shlomai, J., Nurnberg, G., Nurnberg, P., Levy, S., Smogorzewska, A., Otto, E.A., and Hildebrandt, F. (2012) Exome Capture Reveals ZNF423 and CEP164 Mutations, Linking Renal Ciliopathies to DNA Damage Response Signaling. Cell. 150(3):533-548

Zhou, W., and Hildebrandt, F. (2012) Inducible Podocyte Injury and Proteinuria in Transgenic Zebrafish. Journal of the American Society of Nephrology : JASN. 23(6):1039-1047

Hirata, H., Wen, H., Kawakami, Y., Naganawa, Y., Ogino, K., Yamada, K., Saint-Amant, L., Low, S.E., Cui, W.W., Zhou, W., Sprague, S.M., Asakawa, K., Muto, A., Kawakami, K., and Kuwada, J.Y. (2012) Connexin39.9 is necessary for coordinated activation of slow-twitch muscle and normal behavior in zebrafish. The Journal of biological chemistry. 287(2):1080-9

Low, S.E., Amburgey, K., Horstick, E., Linsley, J., Sprague, S.M., Cui, W.W., Zhou, W., Hirata, H., Saint-Amant, L., Hume, R.I., and Kuwada, J.Y. (2011) TRPM7 Is Required within Zebrafish Sensory Neurons for the Activation of Touch-Evoked Escape Behaviors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(32):11633-11644

Heeringa, S.F., Chernin, G., Chaki, M., Zhou, W., Sloan, A.J., Ji, Z., Xie, L.X., Salviati, L., Hurd, T.W., Vega-Warner, V., Killen, P.D., Raphael, Y., Ashraf, S., Ovunc, B., Schoeb, D.S., McLaughlin, H.M., Airik, R., Vlangos, C.N., Gbadegesin, R., Hinkes, B., Saisawat, P., Trevisson, E., Doimo, M., Casarin, A., Pertegato, V., Giorgi, G., Prokisch, H., Rötig, A., Nürnberg, G., Becker, C., Wang, S., Ozaltin, F., Topaloglu, R., Bakkaloglu, A., Bakkaloglu, S.A., Müller, D., Beissert, A., Mir, S., Berdeli, A., Varpizen, S., Zenker, M., Matejas, V., Santos-Ocaña, C., Navas, P., Kusakabe, T., Kispert, A., Akman, S., Soliman, N.A., Krick, S., Mundel, P., Reiser, J., Nürnberg, P., Clarke, C.F., Wiggins, R.C., Faul, C., and Hildebrandt, F. (2011) COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness. J. Clin. Invest.. 121(5):2013-2024

Hurd, T., Zhou, W., Jenkins, P., Liu, C.J., Swaroop, A., Khanna, H., Martens, J., Hildebrandt, F., and Margolis, B. (2010) The Retinitis Pigmentosa Protein RP2 Interacts With Polycystin 2 and Regulates Cilia-Mediated Vertebrate Development. Human molecular genetics. 19(22):4330-4344

Zhou, W., Boucher, R.C., Bollig, F., Englert, C., and Hildebrandt, F. (2010) Characterization of Mesonephric Development and Regeneration Using Transgenic Zebrafish. American journal of physiology. Renal physiology. 299(5):F1040-F1047

Otto, E.A., Hurd, T.W., Airik, R., Chaki, M., Zhou, W., Stoetzel, C., Patil, S.B., Levy, S., Ghosh, A.K., Murga-Zamalloa, C.A., van Reeuwijk, J., Letteboer, S.J., Sang, L., Giles, R.H., Liu, Q., Coene, K.L., Estrada-Cuzcano, A., Collin, R.W., McLaughlin, H.M., Held, S., Kasanuki, J.M., Ramaswami, G., Conte, J., Lopez, I., Washburn, J., Macdonald, J., Hu, J., Yamashita, Y., Maher, E.R., Guay-Woodford, L.M., Neumann, H.P., Obermüller, N., Koenekoop, R.K., Bergmann, C., Bei, X., Lewis, R.A., Katsanis, N., Lopes, V., Williams, D.S., Lyons, R.H., Dang, C.V., Brito, D.A., Dias, M.B., Zhang, X., Cavalcoli, J.D., Nürnberg, G., Nürnberg, P., Pierce, E.A., Jackson, P.K., Antignac, C., Saunier, S., Roepman, R., Dollfus, H., Khanna, H., and Hildebrandt, F. (2010) Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy. Nature Genetics. 42(10):840-850

Low, S.E., Ryan, J., Sprague, S.M., Hirata, H., Cui, W.W., Zhou, W., Hume, R.I., Kuwada, J.Y., and Saint-Amant, L. (2010) touche Is Required for Touch-Evoked Generator Potentials within Vertebrate Sensory Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(28):9359-9367

Zhou, W., Dai, J., Attanasio, M., and Hildebrandt, F. (2010) Nephrocystin-3 is required for ciliary function in zebrafish embryos. American journal of physiology. Renal physiology. 299(1):F55-F62

Low, S.E., Zhou, W., Choong, X., Saint-Amant, L., Sprague, S.M., Hirata, H., Cui, W.W., Hume, R.I., and Kuwada, J.Y. (2010) Na(V)1.6a is required for normal activation of motor circuits normally excited by tactile stimulation. Developmental Neurobiology. 70(7):508-522

O'Toole, J.F., Liu, Y., Davis, E.E., Westlake, C.J., Attanasio, M., Otto, E.A., Seelow, D., Nurnberg, G., Becker, C., Nuutinen, M., Kärppä, M., Ignatius, J., Uusimaa, J., Pakanen, S., Jaakkola, E., van den Heuvel, L.P., Fehrenbach, H., Wiggins, R., Goyal, M., Zhou, W., Wolf, M.T., Wise, E., Helou, J., Allen, S.J., Murga-Zamalloa, C.A., Ashraf, S., Chaki, M., Heeringa, S., Chernin, G., Hoskins, B.E., Chaib, H., Gleeson, J., Kusakabe, T., Suzuki, T., Isaac, R.E., Quarmby, L.M., Tennant, B., Fujioka, H., Tuominen, H., Hassinen, I., Lohi, H., van Houten, J.L., Rotig, A., Sayer, J.A., Rolinski, B., Freisinger, P., Madhavan, S.M., Herzer, M., Madignier, F., Prokisch, H., Nurnberg, P., Jackson, P., Khanna, H., Katsanis, N., and Hildebrandt, F. (2010) Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy. J. Clin. Invest.. 120(3):791-802

Zhou, W., and Hildebrandt, F. (2009) Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish. Gene expression patterns : GEP. 9(5):282-288

He, C., Bartholomew, C.R., Zhou, W., and Klionsky, D.J. (2009) Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos. Autophagy. 5(4):520-526

Zhou, W., Horstick, E.J., Hirata, H., and Kuwada, J.Y. (2008) Identification and expression of voltage-gated calcium channel beta subunits in Zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(12):3842-3852

Saint-Amant, L., Sprague, S.M., Hirata, H., Li, Q., Cui, W.W., Zhou, W., Poudou, O., Hume, R.I., and Kuwada, J.Y. (2008) The zebrafish ennui behavioral mutation disrupts acetylcholine receptor localization and motor axon stability. Developmental Neurobiology. 68(1):45-61

Hirata, H., Watanabe, T., Hatakeyama, J., Sprague, S.M., Saint-Amant, L., Nagashima, A., Cui, W.W., Zhou, W., and Kuwada, J.Y. (2007) Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development (Cambridge, England). 134(15):2771-2781

Zhou, W., Shirabe, K., and Kuwada, J.Y. (2006) Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish. Gene expression patterns : GEP. 6(5):482-488

Zhou, W., Saint-Amant, L., Hirata, H., Cui, W.W., Sprague, S.M., and Kuwada, J.Y. (2006) Non-sense mutations in the dihydropyridine receptor beta1 gene, CACNB1, paralyze zebrafish relaxed mutants. Cell Calcium. 39(3):227-236

Hirata, H., Saint-Amant, L., Downes, G.B., Cui, W.W., Zhou, W., Granato, M., and Kuwada, J.Y. (2005) Zebrafish bandoneon mutants display behavioral defects due to a mutation in the glycine receptor β-subunit. Proceedings of the National Academy of Sciences of the United States of America. 102(23):8345-8350

Hirata, H., Saint-Amant, L., Waterbury, J., Cui, W., Zhou, W., Li, Q., Goldman, D., Granato, M., and Kuwada, J.Y. (2004) accordion, a zebrafish behavioral mutant, has a muscle relaxation defect due to a mutation in the ATPase Ca2+ pump SERCA1. Development (Cambridge, England). 131(21):5457-5468

Xiao, T., Shoji, W., Zhou, W., Su, F., and Kuwada, J.Y. (2003) Transmembrane Sema4E guides branchiomotor axons to their targets in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(10):4190-4198

Masumoto, J., Zhou, W., Chen, F.F., Su, F., Kuwada, J.Y., Hidaka, E., Katsuyama, T., Sagara, J., Taniguchi, S., Ngo-Hazelett, P., Postlethwait, J.H., Nuñez, G., and Inohara, N. (2003) Caspy: A Zebrafish caspase activated by ASC oligomerization required for pharyngeal arch development. The Journal of biological chemistry. 278(6):4268-4276