UniProt ID: Q1RLW7 |
SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}. SIMILARITY: Belongs to the RAG2 family. {ECO:0000256|ARBA:ARBA00008254}. |
UniProt ID: O13034 |
FUNCTION: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T- cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In the RAG complex, rag2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity). {ECO:0000250}. SUBUNIT: Component of the RAG complex composed of core components rag1 and rag2. {ECO:0000250}. SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. DEVELOPMENTAL STAGE: First detected in the thymus during day 4 of development. Expression then increases in the thymus for at least three weeks. {ECO:0000269|PubMed:9070331, ECO:0000269|PubMed:9089097}. DOMAIN: The atypical PHD-type zinc finger recognizes and binds histone H3 trimethylated on 'Lys-4' (H3K4me3). The atypical PHD-type zinc finger also binds various phosphoinositides (By similarity). {ECO:0000250}. SIMILARITY: Belongs to the RAG2 family. {ECO:0000305}. |
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