UniProt ID: A0A8M6Z104 |
SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}. SIMILARITY: Belongs to the hedgehog family. {ECO:0000256|ARBA:ARBA00010649}. |
UniProt ID: Q98862 |
FUNCTION: [Indian hedgehog B protein]: The C-terminal part of the indian hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts followed by the covalent attachment of a cholesterol moiety to the C- terminal of the newly generated N-product (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). {ECO:0000250|UniProtKB:Q62226}. FUNCTION: [Indian hedgehog B protein]: The dually lipidated indian hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). In the notochord, induces somite patterning and muscle pioneer differentiation (PubMed:8684485). {ECO:0000250|UniProtKB:Q15465, ECO:0000250|UniProtKB:Q62226, ECO:0000269|PubMed:8684485}. CATALYTIC ACTIVITY: [Indian hedgehog B protein]: Reaction=cholesterol + glycyl-L-cysteinyl-[protein] + H(+) = [protein]- C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl- [protein]; Xref=Rhea:RHEA:59504, Rhea:RHEA-COMP:12707, Rhea:RHEA- COMP:15369, Rhea:RHEA-COMP:15374, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:65250, ChEBI:CHEBI:143135, ChEBI:CHEBI:143140; Evidence={ECO:0000250|UniProtKB:Q62226}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59505; Evidence={ECO:0000250|UniProtKB:Q62226}; SUBUNIT: [Indian hedgehog B protein N-product]: Multimer. {ECO:0000250|UniProtKB:Q14623}. SUBUNIT: Interacts with BOC and CDON. Interacts with PTCH1 (By similarity). Interacts with glypican GPC3 (By similarity). {ECO:0000250|UniProtKB:P97812, ECO:0000250|UniProtKB:Q14623}. SUBCELLULAR LOCATION: [Indian hedgehog B protein N-product]: Cell membrane {ECO:0000250|UniProtKB:Q14623}; Lipid-anchor {ECO:0000250|UniProtKB:Q62226}. Note=The N-product remains associated with the cell surface. {ECO:0000250|UniProtKB:Q15465}. SUBCELLULAR LOCATION: [Indian hedgehog B protein]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q15465}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q15465}. Secreted {ECO:0000250|UniProtKB:Q14623}. Note=Co-localizes with HHAT in the ER and Golgi membrane. {ECO:0000250|UniProtKB:Q15465}. TISSUE SPECIFICITY: Expressed exclusively in the notochord. {ECO:0000269|PubMed:8684485}. DEVELOPMENTAL STAGE: First detectable at the mid-gastrula stage. Disappears at the end of the somitogenesis. {ECO:0000269|PubMed:8684485}. DOMAIN: [Indian hedgehog B protein N-product]: Binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein- protein interactions mediated by this domain. {ECO:0000250|UniProtKB:Q14623}. PTM: [Indian hedgehog B protein N-product]: Cholesterylation is required for N-product targeting to lipid rafts and multimerization. {ECO:0000250|UniProtKB:Q14623}. PTM: [Indian hedgehog B protein]: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C- terminal of the newly generated N-product (By similarity). The N- product is the active species in both local and long-range signaling, whereas the C-product is degraded in the reticulum endoplasmic (By similarity). {ECO:0000250|UniProtKB:Q15465, ECO:0000250|UniProtKB:Q62226}. PTM: [Indian hedgehog B protein N-product]: N-palmitoylation by HHAT of N-product is required for indian hedgehog protein N-product multimerization and full activity. {ECO:0000250|UniProtKB:Q14623}. SIMILARITY: Belongs to the hedgehog family. {ECO:0000305}. |
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