UniProt ID: F1QBP9
FUNCTION: Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N- glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. {ECO:0000256|HAMAP-Rule:MF_03183}.
CATALYTIC ACTIVITY: Reaction=The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'- terminal unsaturated sugar and a product with a terminal 5'- phosphate.; EC=4.2.99.18; Evidence={ECO:0000256|HAMAP- Rule:MF_03183};
COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000256|HAMAP-Rule:MF_03183}; Note=Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand. {ECO:0000256|HAMAP-Rule:MF_03183};
SUBCELLULAR LOCATION: Nucleus {ECO:0000256|HAMAP-Rule:MF_03183}. Mitochondrion {ECO:0000256|HAMAP-Rule:MF_03183}.
SIMILARITY: Belongs to the Nth/MutY family. {ECO:0000256|HAMAP- Rule:MF_03183}.
This information was provided by UniProt through a collaboration with ZFIN. (1)