UniProt ID: A0A8M9QKQ6 |
FUNCTION: Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. {ECO:0000256|HAMAP-Rule:MF_03048}. PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3. {ECO:0000256|HAMAP-Rule:MF_03048}. SIMILARITY: Belongs to the URM1 family. {ECO:0000256|HAMAP- Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. |
UniProt ID: F2Z4T6 |
FUNCTION: Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. {ECO:0000256|HAMAP-Rule:MF_03048}. PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3. {ECO:0000256|HAMAP-Rule:MF_03048}. SIMILARITY: Belongs to the URM1 family. {ECO:0000256|HAMAP- Rule:MF_03048, ECO:0000256|RuleBase:RU361182}. |
This information was provided by UniProt through a collaboration with ZFIN. (1) |