UniProt ID: E7FDW2 |
FUNCTION: E3 ubiquitin-protein ligase. {ECO:0000250|UniProtKB:Q69ZS0}. CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q69ZS0}; PATHWAY: Protein modification; protein ubiquitination. {ECO:0000250|UniProtKB:Q69ZS0}. SUBUNIT: Interacts (via PDZ domain 1) with kidins220b (via PDZ-binding motif). {ECO:0000269|PubMed:22609016}. SUBCELLULAR LOCATION: Synapse {ECO:0000250|UniProtKB:Q69ZS0}. Cytoplasm {ECO:0000269|PubMed:22609016}. Note=Localizes to the postsynaptic region of neuromuscular junctions. {ECO:0000250|UniProtKB:Q69ZS0}. DEVELOPMENTAL STAGE: Detected in the presumptive hindbrain and notochord from the 3-somite stage onwards (PubMed:22252497). Expression in notochord becomes restricted to the posterior as development proceeds (PubMed:22252497, PubMed:22609016). Shows particularly strong expression at the hindbrain-rhombomere 1 boundary during the 12-18 somite stages (PubMed:22252497, PubMed:22609016). Expressed in ventral spinal cord neurons and ventral somite cells at 24 hours post- fertilization (hpf) (PubMed:22252497). Becomes restricted to trunk-tail motor neurons at 32 hpf (PubMed:22252497, PubMed:22609016). Detected in eye primordia from the 10-somite stage onwards (PubMed:22252497). At 24 hpf, expression in eye is found in the ventral retina and optic stalk, and also weakly in dorsal retina (PubMed:22252497). Expressed in the eye choroid fissure and ganglion cells at 32 hpf (PubMed:22252497, PubMed:22609016). Also found in thalamus, posterior tuberculum, head mesenchyme, pectoral fins and pronephric duct at 32 hpf (PubMed:22252497). {ECO:0000269|PubMed:22252497, ECO:0000269|PubMed:22609016}. DOMAIN: The RING-type zinc finger domain is required for E3 ligase activity. {ECO:0000250|UniProtKB:Q69ZS0}. |
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