UniProt ID: F1QF89 |
FUNCTION: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. {ECO:0000269|PubMed:20466645}. CATALYTIC ACTIVITY: Reaction=a dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3- O-(alpha-D-mannosyl)-L-seryl-[protein] + a dolichyl phosphate + H(+); Xref=Rhea:RHEA:17377, Rhea:RHEA-COMP:9517, Rhea:RHEA-COMP:9527, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:13546, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57683, ChEBI:CHEBI:58211, ChEBI:CHEBI:137321; EC=2.4.1.109; Evidence={ECO:0000269|PubMed:20466645}; CATALYTIC ACTIVITY: Reaction=a dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a dolichyl phosphate + H(+); Xref=Rhea:RHEA:53396, Rhea:RHEA-COMP:9517, Rhea:RHEA-COMP:9527, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:13547, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:57683, ChEBI:CHEBI:58211, ChEBI:CHEBI:137323; EC=2.4.1.109; Evidence={ECO:0000269|PubMed:20466645}; PATHWAY: Protein modification; protein glycosylation. {ECO:0000269|PubMed:20466645}. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Y6A1}; Multi-pass membrane protein {ECO:0000255}. TISSUE SPECIFICITY: Widely expressed. Has particularly strong expression in testis, ovary, brain, liver and heart. {ECO:0000269|PubMed:20466645}. DEVELOPMENTAL STAGE: Detected throughout development (PubMed:18632251, PubMed:20466645). Highest expression levels are found at 0 hours post- fertilization (hpf), probably due to perdurance of maternal transcripts (PubMed:20466645). Ubiquitously expressed during early stages of development (PubMed:18632251, PubMed:20466645). At 16-24 hpf, mainly found in eye, brain and somites (PubMed:18632251, PubMed:20466645). At 30 hpf, has strongest expression in forebrain, cerebellum and hindbrain (PubMed:18632251). {ECO:0000269|PubMed:18632251, ECO:0000269|PubMed:20466645}. DISRUPTION PHENOTYPE: Morpholino knockdown of the protein results in developmental delays at 18 hours post fertilization (hpf). At 48-72 hpf, the tail is slightly curved and somite boundaries are also abnormally curved. Swim bladders are incompletely formed. Glycosylation of alpha-dystroglycan (dag1) is severely reduced. {ECO:0000269|PubMed:20466645}. SIMILARITY: Belongs to the glycosyltransferase 39 family. {ECO:0000305}. |
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