UniProt ID: Q08BV2 |
FUNCTION: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a- amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. {ECO:0000250|UniProtKB:A2RUC4}. CATALYTIC ACTIVITY: Reaction=2-oxoglutarate + 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + O2 = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + CO2 + succinate; Xref=Rhea:RHEA:37899, Rhea:RHEA- COMP:10379, Rhea:RHEA-COMP:11848, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, ChEBI:CHEBI:73543, ChEBI:CHEBI:73603; EC=1.14.11.42; Evidence={ECO:0000250|UniProtKB:A2RUC4}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37900; Evidence={ECO:0000250|UniProtKB:A2RUC4}; COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250|UniProtKB:A2RUC4}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250|UniProtKB:A2RUC4}; PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis. {ECO:0000250|UniProtKB:A2RUC4}. SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:A2RUC4}. SIMILARITY: Belongs to the TYW5 family. {ECO:0000305}. |
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