UniProt ID: E7EZ59 |
FUNCTION: Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit. {ECO:0000256|HAMAP-Rule:MF_03056}. PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03056}. SUBUNIT: Forms a heterodimer with the catalytic subunit METTL1. {ECO:0000256|HAMAP-Rule:MF_03056}. SUBCELLULAR LOCATION: Nucleus {ECO:0000256|HAMAP-Rule:MF_03056}. SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000256|HAMAP- Rule:MF_03056}. |
UniProt ID: A4IGH4 |
FUNCTION: Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, wdr4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'- RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs. {ECO:0000255|HAMAP-Rule:MF_03056}. PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03056}. SUBUNIT: Non-catalytic component of the METTL1-WDR4 complex, composed of mettl1 and wdr4. {ECO:0000255|HAMAP-Rule:MF_03056}. SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}. SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP- Rule:MF_03056}. SEQUENCE CAUTION: Sequence=AAI35102.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
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