UniProt ID: Q4KMK0 |
FUNCTION: Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt- tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5- formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. {ECO:0000250|UniProtKB:Q9H649}. CATALYTIC ACTIVITY: Reaction=cytidine(34) in mitochondrial tRNA + S-adenosyl-L-methionine = 5-methylcytidine(34) in mitochondrial tRNA + H(+) + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:53076, Rhea:RHEA-COMP:13451, Rhea:RHEA- COMP:13453, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74483, ChEBI:CHEBI:82748; Evidence={ECO:0000250|UniProtKB:Q9H649}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:53077; Evidence={ECO:0000250|UniProtKB:Q9H649}; SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250|UniProtKB:Q9H649}. SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. {ECO:0000255|PROSITE-ProRule:PRU01023}. SEQUENCE CAUTION: Sequence=AAH76487.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CR762462; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CU915566; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
UniProt ID: A0A8M2B6P7 |
SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}. |
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