UniProt ID: A9C3R9 |
FUNCTION: Specifically generates 13-cis retinol, a stereoisomeric form of retinoic acid. Capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (By similarity). {ECO:0000250|UniProtKB:Q16518, ECO:0000269|PubMed:21235714}. CATALYTIC ACTIVITY: Reaction=an all-trans-retinyl ester + H2O = 13-cis-retinol + a fatty acid + H(+); Xref=Rhea:RHEA:31779, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:45479, ChEBI:CHEBI:63410; EC=3.1.1.90; Evidence={ECO:0000269|PubMed:21235714}; CATALYTIC ACTIVITY: Reaction=lutein = (3R,3'S)-zeaxanthin; Xref=Rhea:RHEA:12729, ChEBI:CHEBI:28838, ChEBI:CHEBI:138919; EC=5.3.3.22; Evidence={ECO:0000250|UniProtKB:Q16518}; COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000305|PubMed:21235714}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000305|PubMed:21235714}; BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.6 uM for all-trans-retinyl ester {ECO:0000269|PubMed:21235714}; Note=kcat is 0.00044 sec(-1) with all-trans-retinyl ester as substrate.; SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21235714}. Cell membrane {ECO:0000269|PubMed:21235714}; Lipid-anchor {ECO:0000269|PubMed:21235714}. Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. {ECO:0000250|UniProtKB:Q28175}. TISSUE SPECIFICITY: Predominantly expressed in brain. Expressed at a low level in the eye. {ECO:0000269|PubMed:21235714}. DEVELOPMENTAL STAGE: Detectable in migrating neural crest cells during somitogenesis. At early larval stages, expression is visible in the ventricular zone, the upper and lower jaw and the developing pectoral fins. At later larval stages, expression fades out. {ECO:0000269|PubMed:17868371}. PTM: Palmitoylated. {ECO:0000250|UniProtKB:Q28175}. SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}. |
UniProt ID: A0A8M9QID4 |
COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000256|ARBA:ARBA00001954}; SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000256|ARBA:ARBA00006787, ECO:0000256|RuleBase:RU003799}. |
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