UniProt ID: Q66L58 |
FUNCTION: Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped structure and nucleates the assembly of the complex. The pentamer binds a single copy each of PIKFYVE and FIG4 and coordinates both PIKfyve kinase activity and FIG4 phosphatase activity, being required to maintain normal levels of phosphatidylinositol 3- phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. {ECO:0000250|UniProtKB:Q08AM6}. SUBUNIT: Forms pentamers. Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold by pentamerizing into a star-shaped structure, which can bind a single copy each of PIKFYVE and FIG4 and coordinates their activities. Interacts with NOS1. {ECO:0000250|UniProtKB:Q08AM6}. SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250|UniProtKB:Q08AM6}. Microsome membrane {ECO:0000250|UniProtKB:Q80W92}. Note=Mainly associated with membranes of the late endocytic pathway. {ECO:0000250|UniProtKB:Q08AM6}. DOMAIN: The C-terminal domain (residues 523-782) mediates pentameric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex. {ECO:0000250|UniProtKB:Q08AM6}. SIMILARITY: Belongs to the VAC14 family. {ECO:0000305}. |
UniProt ID: A0A0R4ITZ2 |
SUBCELLULAR LOCATION: Endosome membrane {ECO:0000256|ARBA:ARBA00004608}. Microsome membrane {ECO:0000256|ARBA:ARBA00004524}. SIMILARITY: Belongs to the VAC14 family. {ECO:0000256|ARBA:ARBA00010225}. |
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