UniProt ID: Q1JPY5
FUNCTION: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. {ECO:0000269|PubMed:24361620}.
CATALYTIC ACTIVITY: Reaction=a 14alpha-methyl steroid + 3 O2 + 3 reduced [NADPH-- hemoprotein reductase] = a Delta(14) steroid + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:54028, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:138029, ChEBI:CHEBI:138031; EC=; Evidence={ECO:0000269|PubMed:24361620};
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250|UniProtKB:Q16850};
ACTIVITY REGULATION: Inhibited by ketoconazole. May also be inhibited to a lesser extent by propiconazole. {ECO:0000269|PubMed:24361620}.
PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6. {ECO:0000305|PubMed:24361620}.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein {ECO:0000255}. Membrane {ECO:0000305|PubMed:24361620}; Single-pass membrane protein {ECO:0000255}.
TISSUE SPECIFICITY: Strongly expressed in intestine. Moderately expressed in liver, with higher levels in females compared to males. Also detected at low levels in brain, eye, kidney and testis. {ECO:0000269|PubMed:24361620}.
SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000255|RuleBase:RU000461, ECO:0000305}.
This information was provided by UniProt through a collaboration with ZFIN. (1)