UniProt ID: F1RCY6 |
FUNCTION: RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. The formation of an upf1-upf2-upf3 surveillance complex is believed to activate NMD. {ECO:0000250|UniProtKB:Q92900}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9EPU0}. Cytoplasm, P-body {ECO:0000250|UniProtKB:Q92900}. Nucleus {ECO:0000250|UniProtKB:Q92900}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q9EPU0}. DEVELOPMENTAL STAGE: Expressed during early cleavage, gastrulation and at 1 day post-fertilization. {ECO:0000269|PubMed:19414594}. DISRUPTION PHENOTYPE: Morpholino knockdown leads to both intermediate and severe phenotypes of abnormal embryonic development (PubMed:21227923, PubMed:19414594). Intermediate phenotypes show developmental delay, including aberrant formation of the head, tail, eye placodes and somites (PubMed:21227923, PubMed:19414594). Severe phenotypes show severe developmental arrest, including yolk extension defects, and lack of recognizable morphological development (PubMed:21227923, PubMed:19414594). High mortality rates of 80% to 85% at 5 days post-fertilization (PubMed:19414594). Increase in premature stop codon containing slc24a5 mRNA transcript levels in melanocytes (PubMed:21227923, PubMed:19414594). {ECO:0000269|PubMed:19414594, ECO:0000269|PubMed:21227923}. SIMILARITY: Belongs to the DNA2/NAM7 helicase family. {ECO:0000250|UniProtKB:Q92900}. |
UniProt ID: A0A8M2BAV2 |
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. SIMILARITY: Belongs to the DNA2/NAM7 helicase family. {ECO:0000256|ARBA:ARBA00007913}. |
This information was provided by UniProt through a collaboration with ZFIN. (1) |