UniProt ID: A0A8M2B283 |
CATALYTIC ACTIVITY: Reaction=ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L- cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.; EC=6.2.1.64; Evidence={ECO:0000256|ARBA:ARBA00024626}; PATHWAY: Protein modification; protein neddylation. {ECO:0000256|ARBA:ARBA00005032}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. {ECO:0000256|ARBA:ARBA00006310}. |
UniProt ID: Q7ZVX6 |
FUNCTION: Catalytic subunit of the dimeric uba3-nae1 E1 enzyme. E1 activates nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1 finally transfers nedd8 to the catalytic cysteine of ube2m (By similarity). {ECO:0000250}. CATALYTIC ACTIVITY: Reaction=ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L- cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.; EC=6.2.1.64; PATHWAY: Protein modification; protein neddylation. SUBUNIT: Heterodimer of uba3 and nae1. Interacts with nedd8, ube2f and ube2m (By similarity). {ECO:0000250}. MISCELLANEOUS: Arg-210 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex. {ECO:0000250}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. {ECO:0000305}. |
UniProt ID: B2GSP4 |
CATALYTIC ACTIVITY: Reaction=ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L- cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.; EC=6.2.1.64; Evidence={ECO:0000256|ARBA:ARBA00024626}; PATHWAY: Protein modification; protein neddylation. {ECO:0000256|ARBA:ARBA00005032}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. {ECO:0000256|ARBA:ARBA00006310}. |
This information was provided by UniProt through a collaboration with ZFIN. (1) |