UniProt ID: A0A8M3ANZ0 |
FUNCTION: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000256|PIRNR:PIRNR039133}. PATHWAY: Protein modification; protein sumoylation. {ECO:0000256|ARBA:ARBA00004718, ECO:0000256|PIRNR:PIRNR039133}. SUBUNIT: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. {ECO:0000256|ARBA:ARBA00026003}. SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR039133}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. {ECO:0000256|ARBA:ARBA00005673, ECO:0000256|PIRNR:PIRNR039133}. |
UniProt ID: Q7SXG4 |
FUNCTION: The heterodimer acts as an E1 ligase for sumo1, sumo2, and sumo3. It mediates ATP-dependent activation of sumo proteins followed by formation of a thioester bond between a sumo protein and a conserved active site cysteine residue on uba2/sae2 (By similarity). {ECO:0000250|UniProtKB:Q9UBT2}. PATHWAY: Protein modification; protein sumoylation. SUBUNIT: Heterodimer of sae1 and uba2/sae2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with ube2i (By similarity). {ECO:0000250}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention. {ECO:0000250}. TISSUE SPECIFICITY: Expressed in eye, brain and pectoral fins. {ECO:0000269|PubMed:34040189}. PTM: Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys- 237 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking (By similarity). {ECO:0000250}. DISRUPTION PHENOTYPE: Knockout animals show deficient growth, microcephaly, microphthalmia, mandibular hypoplasia and abnormal fins. {ECO:0000269|PubMed:34040189}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. {ECO:0000305}. |
UniProt ID: B2GQ09 |
FUNCTION: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000256|PIRNR:PIRNR039133}. PATHWAY: Protein modification; protein sumoylation. {ECO:0000256|ARBA:ARBA00004718, ECO:0000256|PIRNR:PIRNR039133}. SUBUNIT: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. {ECO:0000256|ARBA:ARBA00026003}. SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR039133}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. {ECO:0000256|ARBA:ARBA00005673, ECO:0000256|PIRNR:PIRNR039133}. |
UniProt ID: A0A8M3AWE5 |
FUNCTION: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000256|PIRNR:PIRNR039133}. PATHWAY: Protein modification; protein sumoylation. {ECO:0000256|ARBA:ARBA00004718, ECO:0000256|PIRNR:PIRNR039133}. SUBUNIT: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. {ECO:0000256|ARBA:ARBA00026003}. SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR039133}. SIMILARITY: Belongs to the ubiquitin-activating E1 family. {ECO:0000256|ARBA:ARBA00005673, ECO:0000256|PIRNR:PIRNR039133}. |
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