UniProt ID: Q6QA32 |
FUNCTION: Catalyzes the conversion of 17-oxosteroids to 17beta- hydroxysteroids in the presence of NADPH (PubMed:16216911, PubMed:27927697). Favors the reduction of androstenedione to testosterone (PubMed:16216911, PubMed:27927697). Testosterone is the key androgen driving male development and function (By similarity). Among further tested androgens epiandrosterone and dehydroepiandrosterone are accepted as substrates and reduced at C-17 (PubMed:16216911). Can also reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed:16216911, PubMed:27927697). Cannot use androsterone and androstanedione as substrates (PubMed:16216911). {ECO:0000250|UniProtKB:P70385, ECO:0000269|PubMed:16216911, ECO:0000269|PubMed:27927697}. CATALYTIC ACTIVITY: Reaction=a 17beta-hydroxy steroid + NADP(+) = a 17-oxo steroid + H(+) + NADPH; Xref=Rhea:RHEA:69284, ChEBI:CHEBI:15378, ChEBI:CHEBI:19168, ChEBI:CHEBI:35343, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:16216911, ECO:0000269|PubMed:27927697}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:69286; Evidence={ECO:0000305|PubMed:16216911, ECO:0000305|PubMed:27927697}; CATALYTIC ACTIVITY: Reaction=NADP(+) + testosterone = androst-4-ene-3,17-dione + H(+) + NADPH; Xref=Rhea:RHEA:14981, ChEBI:CHEBI:15378, ChEBI:CHEBI:16422, ChEBI:CHEBI:17347, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.64; Evidence={ECO:0000269|PubMed:16216911, ECO:0000269|PubMed:27927697}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:14983; Evidence={ECO:0000305|PubMed:16216911, ECO:0000305|PubMed:27927697}; CATALYTIC ACTIVITY: Reaction=3beta-hydroxyandrost-5-en-17-one + H(+) + NADPH = androst-5- en-3beta,17beta-diol + NADP(+); Xref=Rhea:RHEA:46628, ChEBI:CHEBI:2710, ChEBI:CHEBI:15378, ChEBI:CHEBI:28689, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:16216911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46629; Evidence={ECO:0000305|PubMed:16216911}; CATALYTIC ACTIVITY: Reaction=3beta-hydroxy-5alpha-androstan-17-one + H(+) + NADPH = 5alpha- androstane-3beta,17beta-diol + NADP(+); Xref=Rhea:RHEA:53480, ChEBI:CHEBI:15378, ChEBI:CHEBI:18329, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:541975; Evidence={ECO:0000269|PubMed:16216911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:53481; Evidence={ECO:0000305|PubMed:16216911}; CATALYTIC ACTIVITY: Reaction=androst-4-ene-3,11,17-trione + H(+) + NADPH = 17beta- hydroxyandrost-4-ene-3,11-dione + NADP(+); Xref=Rhea:RHEA:53484, ChEBI:CHEBI:2495, ChEBI:CHEBI:15378, ChEBI:CHEBI:34133, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:16216911, ECO:0000269|PubMed:27927697}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:53485; Evidence={ECO:0000305|PubMed:16216911, ECO:0000305|PubMed:27927697}; CATALYTIC ACTIVITY: Reaction=11beta-hydroxyandrost-4-ene-3,17-dione + H(+) + NADPH = 11beta,17beta-dihydroxyandrost-4-ene-3-one + NADP(+); Xref=Rhea:RHEA:53488, ChEBI:CHEBI:15378, ChEBI:CHEBI:27967, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:81481; Evidence={ECO:0000269|PubMed:16216911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:53489; Evidence={ECO:0000305|PubMed:16216911}; PATHWAY: Hormone biosynthesis; testosterone biosynthesis. {ECO:0000305}. PATHWAY: Steroid metabolism. {ECO:0000305}. SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:16216911}. Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. TISSUE SPECIFICITY: Expression shows strong sexual dimorphism. In female, highly expressed in ovaries, and at lower levels in skin muscle, eyes and liver (PubMed:16216911). In males, strongly expressed in liver and at lower levels in testis, spleen, kidney, intestine and muscle (PubMed:16216911). {ECO:0000269|PubMed:16216911}. DEVELOPMENTAL STAGE: Expressed in embryogenesis from sphere to 84 h post-fertilization. {ECO:0000269|PubMed:16216911}. SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000255|RuleBase:RU000363}. |
UniProt ID: Q7T396 |
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000256|RuleBase:RU000363}. |
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