UniProt ID: Q66HV4 |
FUNCTION: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin- coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity). {ECO:0000250|UniProtKB:P23514}. SUBUNIT: Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250|UniProtKB:A0JN39}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P23514}. Golgi apparatus membrane {ECO:0000250|UniProtKB:P23514}; Peripheral membrane protein {ECO:0000250|UniProtKB:P23514}; Cytoplasmic side {ECO:0000250|UniProtKB:P23514}. Cytoplasmic vesicle, COPI-coated vesicle membrane {ECO:0000250|UniProtKB:P23514}; Peripheral membrane protein {ECO:0000250|UniProtKB:P23514}; Cytoplasmic side {ECO:0000250|UniProtKB:P23514}. Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. {ECO:0000250|UniProtKB:P23514}. DISRUPTION PHENOTYPE: From 28 hours post-fertilization (hpf) onward, embryos show an anterior-posterior gradient of pigmentation defects. While the head is fully pigmented, more posterior regions are decreasingly pigmented until midtrunk levels, where pigmentation is not detectable. Melanophores are present at midtrunk levels and migrate correctly, but are not properly pigmented. Embryos show severe reduction in pigmentation and widespread degeneration from 48 hpf onward. Failure to form perinotochordal basement membrane (PBM), without the loss of laminin immunoreactivity. Embryos are significantly shorter, because the notochord cells fail to differentiate and the notochord fails to lengthen properly. Vacuoles fail to inflate fully. Many notochord cells die by apoptosis. In 32 hpf embryos the endoplasmic reticulum is abnormally swollen and filled with a relatively electron dense material and the Golgi complex is fragmented into large vesicles throughout the cytoplasm. PBM is thin, disorganized and essentially lacks the medial layer. However, cells have a distinctive inner layer. {ECO:0000269|PubMed:15469843}. |
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