UniProt ID: Q7SXS7
FUNCTION: Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73'. Does not have protein- lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin. {ECO:0000250|UniProtKB:Q86TU7}.
CATALYTIC ACTIVITY: Reaction=L-histidyl-[protein] + S-adenosyl-L-methionine = H(+) + N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:19369, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:11600, ChEBI:CHEBI:15378, ChEBI:CHEBI:16367, ChEBI:CHEBI:29979, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.85; Evidence={ECO:0000250|UniProtKB:Q86TU7};
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86TU7}.
DOMAIN: The SET domain specifically recognizes and binds actin, suggesting that it does not accommodate substrates diverging from actin. {ECO:0000250|UniProtKB:Q86TU7}.
SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily. {ECO:0000255|PROSITE-ProRule:PRU00898}.
CAUTION: Was initially reported to have histone methyltransferase activity and methylate 'Lys-36' of histone H3 (H3K36me) (PubMed:21307598). However, this conclusion was based on mass spectrometry data wherin mass shifts were inconsistent with a bona fide methylation event and the histone methyltransferase activity could not be confirmed (By similarity). {ECO:0000250|UniProtKB:Q86TU7, ECO:0000269|PubMed:21307598}.
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