UniProt ID: Q06ZW3
FUNCTION: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. During early development, required to specify the left-right axis by repressing expression of FGF8, leading to negatively regulate the dorsal organizer formation. {ECO:0000269|PubMed:20133783}.
CATALYTIC ACTIVITY: Reaction=L-lysyl-[histone] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:10024, Rhea:RHEA-COMP:9845, Rhea:RHEA-COMP:9846, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; EC=;
SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Expressed throughout embryogenesis. {ECO:0000269|PubMed:20133783}.
DOMAIN: In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. {ECO:0000250}.
DISRUPTION PHENOTYPE: Randomization of left-right asymmetry, including heart and visceral organs. {ECO:0000269|PubMed:20133783}.
SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. {ECO:0000255|PROSITE- ProRule:PRU00190}.
UniProt ID: E7F7L1
SUBCELLULAR LOCATION: Chromosome {ECO:0000256|SAAS:SAAS00563877}. Nucleus {ECO:0000256|SAAS:SAAS00563921}.
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