UniProt ID: F1QGW6 |
FUNCTION: Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity). {ECO:0000250|UniProtKB:P05198}. CATALYTIC ACTIVITY: Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.3; Evidence={ECO:0000250|UniProtKB:P32481}; SUBUNIT: eIF2 is an heterotrimer composed of an alpha (EIF2S1), a beta (EIF2S2) and a gamma (EIF2S3) chain. eIF2 is member of the 43S pre- initiation complex (43S PIC). {ECO:0000250|UniProtKB:P41091}. DISRUPTION PHENOTYPE: Morpholino knockdown of eif2s3 produces morphants characterized by hypomotility and morphological deficits at 2 dpf. They are shorter with a curved tail. They also display a significant reduction of head size and small eyes. {ECO:0000269|PubMed:27333055}. SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily. {ECO:0000255|PROSITE-ProRule:PRU01059}. |
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