UniProt ID: E7F3I6 |
FUNCTION: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. {ECO:0000269|PubMed:24766810}. CATALYTIC ACTIVITY: Reaction=a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + ADP + H(+); Xref=Rhea:RHEA:15669, Rhea:RHEA-COMP:13180, Rhea:RHEA-COMP:13184, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:136412, ChEBI:CHEBI:136416, ChEBI:CHEBI:456216; EC=2.7.1.78; CATALYTIC ACTIVITY: Reaction=a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'- monophospho-ribonucleoside-RNA + ADP + H(+); Xref=Rhea:RHEA:54580, Rhea:RHEA-COMP:13935, Rhea:RHEA-COMP:13936, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:138282, ChEBI:CHEBI:138284, ChEBI:CHEBI:456216; EC=2.7.1.78; SUBUNIT: Component of the tRNA splicing endonuclease complex. Component of pre-mRNA cleavage complex II (CF-II) (By similarity). {ECO:0000250}. SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. TISSUE SPECIFICITY: Strong neural expression. {ECO:0000269|PubMed:24766810}. DISRUPTION PHENOTYPE: Lethality before 5 days post-fertilization (dpf). Mutants display abnormal swimming behavior, abnormal head shape and curved tail. Defects are due to neuromotor defects and cerebellar neurodegeneration. {ECO:0000269|PubMed:24766810}. SIMILARITY: Belongs to the Clp1 family. Clp1 subfamily. {ECO:0000305}. |
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