UniProt ID: Q7SXR3 |
FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin- protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (By similarity). Plays a role in erythroblast maturation and in the development of mature macrophages (By similarity). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (By similarity). Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity). May contribute to nuclear architecture and cells division events (By similarity). {ECO:0000250|UniProtKB:Q4VC33, ECO:0000250|UniProtKB:Q7L5Y9}. CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q7L5Y9}; SUBUNIT: Identified in the CTLH complex that contains at least MAEA, RMND5A, GID8, WDR26, and RANBP9 and/or RANBP10 as the catalytic core. Interacts with F-actin. {ECO:0000250|UniProtKB:Q7L5Y9}. SUBCELLULAR LOCATION: Nucleus matrix {ECO:0000250|UniProtKB:Q4VC33}. Cell membrane {ECO:0000250|UniProtKB:Q4VC33}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q4VC33}. Note=Migration from nuclear matrix in immature macrophages to cell surface in mature ones. {ECO:0000250|UniProtKB:Q4VC33}. DOMAIN: The expected RING-type zinc finger domain is highly divergent and most of the expected Cys residues are not conserved. Still, the protein is required for CTLH complex E3 ubiquitin-protein transferase activity. In addition, the conserved Cys-314 in this highly divergent region is required for ubiquitination by the yeast GID complex, suggesting a direct role in catalyzing ubiquitination. {ECO:0000250|UniProtKB:Q7L5Y9}. SEQUENCE CAUTION: Sequence=AAH55388.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
UniProt ID: A0A8M9QG98 |
CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900}; |
This information was provided by UniProt through a collaboration with ZFIN. (1) |