UniProt ID: A0A0R4I9F4 |
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000256|PIRSR:PIRSR602403-1}; SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|RuleBase:RU000461}. |
UniProt ID: Q6EIG3 |
FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals (PubMed:15661642). RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (By similarity). Catalyzes the hydroxylation of atRA primarily at C-4 and C-18, thereby contributing to the regulation of atRA homeostasis and signaling (By similarity). Hydroxylation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination (By similarity). Involved in the convertion of atRA to all- trans-4-oxo-RA. Can oxidize all-trans-13,14-dihydroretinoate (DRA) to metabolites which could include all-trans-4-oxo-DRA, all-trans-4- hydroxy-DRA, all-trans-5,8-epoxy-DRA, and all-trans-18-hydroxy-DRA (By similarity). Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints (PubMed:22019272). {ECO:0000250|UniProtKB:Q811W2, ECO:0000250|UniProtKB:Q9NR63, ECO:0000269|PubMed:15661642, ECO:0000269|PubMed:22019272}. CATALYTIC ACTIVITY: Reaction=all-trans-retinoate + O2 + reduced [NADPH--hemoprotein reductase] = all-trans-4-hydroxyretinoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:51984, Rhea:RHEA- COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:35291, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:134178; Evidence={ECO:0000250|UniProtKB:Q811W2}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51985; Evidence={ECO:0000250|UniProtKB:Q811W2}; CATALYTIC ACTIVITY: Reaction=all-trans-retinoate + O2 + reduced [NADPH--hemoprotein reductase] = all-trans-18-hydroxyretinoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:55856, Rhea:RHEA- COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:35291, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:139258; Evidence={ECO:0000250|UniProtKB:Q811W2}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55857; Evidence={ECO:0000250|UniProtKB:Q811W2}; COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250|UniProtKB:Q9Y6A2}; SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O43174}; Peripheral membrane protein {ECO:0000250|UniProtKB:O43174}. Microsome membrane {ECO:0000250|UniProtKB:O43174}; Peripheral membrane protein {ECO:0000250|UniProtKB:O43174}. DEVELOPMENTAL STAGE: Expressed zygotically. Weak expression at 75% epiboly. At the 2-somite stage, expressed in the presumptive rhombomere 4 (r4) region partially overlapping with the r3 region. At the 10- somite stage, expressed in the r4, r3 and r2 regions. At the 14-somite stage, expression extends to the r5 and r6 regions and is observed in the diencephalon. At 24-48 hours post-fertilization (hpf), additionally expressed in the eye, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia. {ECO:0000269|PubMed:15661642}. SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000255}. |
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