UniProt ID: Q90WW1 |
FUNCTION: RNA-binding protein which binds to RNA with no sequence specificity (PubMed:20948543). Probably represses translation of specific mRNAs (By similarity). Essential for the development of primordial germ cells (PGCs) by ensuring their proper migration and survival but is not required for PGC specification (PubMed:11691838, PubMed:17418113). Also required to maintain oocyte production in the adult ovary (PubMed:17418113, PubMed:23228893). Negatively regulates phosphorylation of myosin mylpfa/mylz2 (PubMed:20673786). {ECO:0000250|UniProtKB:P60323, ECO:0000269|PubMed:11691838, ECO:0000269|PubMed:17418113, ECO:0000269|PubMed:20673786, ECO:0000269|PubMed:20948543, ECO:0000269|PubMed:23228893}. SUBUNIT: Interacts (via C-terminus) with myosin mylpfa/mylz2; the interaction negatively regulates mylpfa phosphorylation. {ECO:0000269|PubMed:20673786}. SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11691838}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:11691838}. TISSUE SPECIFICITY: In the embryo, displays early ubiquitous expression before being restricted to primordial germ cells in a 3'-UTR-dependent manner (PubMed:11691838). Expressed in early stage germ cells in larval and adult ovaries (PubMed:17418113). {ECO:0000269|PubMed:11691838, ECO:0000269|PubMed:17418113}. DEVELOPMENTAL STAGE: Present in oocytes and in embryos at the 4- and 256-cell stages before the mid-blastula transition (PubMed:11691838). Levels decline and are barely detectable by the fifth day of development (PubMed:11691838). At 21 and 35 days post-fertilization, expression is restricted to perinuclear stage oocytes (PubMed:17418113). Expressed throughout most stages of oocyte development with the highest levels restricted to stage Ib oocytes (PubMed:17418113). {ECO:0000269|PubMed:11691838, ECO:0000269|PubMed:17418113}. DOMAIN: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. {ECO:0000255|PROSITE- ProRule:PRU00855}. DISRUPTION PHENOTYPE: Morpholino knockdown results in severe defects in primordial germ cell development, leading to a reduced number of cells and to migration defects, while development of the soma remains largely unaffected. {ECO:0000269|PubMed:11691838}. SIMILARITY: Belongs to the nanos family. {ECO:0000255|PROSITE- ProRule:PRU00855}. |
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