Expression of GFP in injected and transgenic embryos. Injected F0 embryos were observed by epifluorescence microscopy at 5 (B) and 24 hr (C), compared to uninjected embryos (5 hr, A). F1 embryos from lines XIG-3 (D) and XIG-2 (E and F) were observed alongside nontransgenic siblings at 24 (D), 32 (E), and 72 hr (F) postfertilization. An F1 embryo from line XIG-5 was observed alongside a nontransgenic sibling at 24 hr (G). Chorions were removed from the embryos with pronase in A-C, E-G.

Acknowledgments
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Reprinted from Developmental Biology, 171, Amsterdam, A., Lin, S., and Hopkins, N., The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos, 123-129, Copyright (1995) with permission from Elsevier. Full text @ Dev. Biol.