Altered alcian blue+ goblet cell homeostasis in il10 mutant larvae is rescued after il10 mRNA administration.
a Experimental strategy for il10 rescue experiments and expression levels of il10 mRNA at 5dpf. b Whole-body expression levels of il17a/f3 and ifng1 by qRT-PCR. Each dot represents a pool of 5–10 larvae collected in three independent experiments. c Alcian blue stainings on 5dpf WT, il10-Mut larva controls and injected with il10 mRNA. Scale bar = 100 µm. d Quantification for the ab-stained area in the il10 mRNA-injected il10-KO larvae, compared to control il10-KO and WT larvae. N = 2 independent experiments. e Experimental strategy for the injection and analysis of il17 (il17a/f3) mRNA-injected WT zebrafish embryos. f Whole-body expression levels of il17a/f3 and il10 by qPCR. Dots represent pools of 5–10 larvae collected in 2 independent experiments. g Alcian blue stainings on 5dpf control and il17-injected WT larvae. Scale bar = 100 µm. h Quantification of the ab-stained area of control and il17-injected larvae. N = 2 independent experiments. Each independent experiment is shown with a different symbol shape in a, b, d, f and h. One-way ANOVA was used for statistical analysis in a, b and d, whereas two-tailed student t-test were used in f and h (*p < 0.05; ***p < 0.001).