- Title
-
Multi-Dimensional Transcriptome Analysis Reveals Modulation of Cholesterol Metabolism as Highly Integrated Response to Brain Injury
- Authors
- Gourain, V., Armant, O., Lübke, L., Diotel, N., Rastegar, S., Strähle, U.
- Source
- Full text @ Front. Neurosci.
Injury-induced changes in level of polyadenylated RNAs. |
Selected enriched biological functions. To gain information about functions of genes with significant changes in their levels of transcript, the enrichment of ontologies (GO terms), signaling pathways (KEGG pathways) and metabolism pathways (Reactome pathways) was tested and up-regulated genes (red) and down-regulated genes (green) were detailed. *adjp = 0.05, **adjp < 0.01, ***adjp < 0.001 (For complete list see |
Alteration in cholesterol metabolism in response to brain injury. |
Sterol Regulatory Element (SRE) motif analysis. |
Injury-induced changes in levels of miRNAs. (A) The consistency of small RNASeq samples was tested by hierarchical clustering on Euclidean distances as for the RNASeq samples (see Figure 1A). The small RNASeq samples were consistently grouped into their respective condition, control or injured. (B) Changes in level of miRNAs were assessed comparing injured and uninjured telencephalic hemispheres. Significant differences in level of miRNA were tested (red; adjp < 0.05). (C) Targets of miR-182, miR155, miR-146a, and miR-31 were identified in the cholesterol synthesis pathway. For further details see also legend to Figures 3A, 4C. |
Differentially expressed lncRNAs selected for their association with cholesterol synthesis or transport. LncRNAs annotated in the zebrafish genome, and with significant changes in their respective levels upon injury, were localized in the direct vicinity of genes encoding cholesterol-related proteins. Color indicates pairs of coding and non-coding RNAs. *adjp = 0.05, **adjp < 10– 02, ***adjp < 10– 04, ns: not significant. See legend of |
Alternative splicing of RNAs related to cholesterol metabolism in response to injury. |