Bassi et al., 2020 - Knocking-down of the Prokineticin receptor 2 affects reveals its complex role in the regulation of the hypothalamus-pituitary-gonadal axis in the zebrafish model. Scientific Reports   10:7632 Full text @ Sci. Rep.

Fig. 1 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Shield to Day 4

Fig. 2 Whole-mount in situ hybridization with prokr1a probe (A–F) did not display any signal in the head of zebrafsh embryos. In contrast, prokr1b (G–L) is expressed in cells near the olfactory region at 36 hpf (G,H), 48 hpf (I,J) and 72 hpf (K,L). Tat is similar to gnrh3 expression at these times (M–R). Panels A,C,E,G,I,K,M,O and Q show lateral views. Panels B,D,F,H,J,L,N,P and R show dorsal views of zebrafsh embryos. Scale bar=50µm. Image taken using Leica Application Suite sofware

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-25 to Protruding-mouth

Fig. 3 Injection of ctrl-MO (B) or prokr1a-MO (C) does not afect GnRH3 neuron fbers, as they look comparable to uninjected WT (A) embryos. In contrast, knockdown of prokr1b (D) causes alteration in axons migration of the rostral part (dotted square in Fig A) and at the level of the anterior commissure (AC) of the embryos. OB=olfactory bulbs, OC=optic chiasm, AC=anterior commissure. Scale bar=50 µm.

Fig. 4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 5 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 6 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.