Isles et al., 2019 - The CXCL12/CXCR4 Signaling Axis Retains Neutrophils at Inflammatory Sites in Zebrafish. Frontiers in immunology   10:1784 Full text @ Front Immunol

Fig. 1

cxcr4b and cxcl12a are expressed following tissue damage in zebrafish. (A–D) Single-cell gene expression profiles for cxcr4 and cxcl12 in the zebrafish blood lineage. Single cell gene expression values extracted from the Sanger BASiCz zebrafish blood atlas. Circles represent individual cells color coded where red is high expression and yellow is no expression. Neutrophil lineage (mpx:GFP positive) is highlighted by black dashed box and expanded in (i–iv). (E,F) RNA sequencing of FACS sorted GFP positive cells from TgBAC(mpx:GFP)i114 zebrafish larvae at 5 days post fertilization. FPKM values illustrate neutrophil expression of (E) cxcr4a and cxcr4b and (F)cxcl12a and cxcl12b(G) Whole mount in situ hybridization using an antisense DIG labeled RNA probe for cxcl12a mRNA. Wildtype nacre zebrafish larvae were injured and fixed in PFA at 6, 12, and 24 h post injury, along with uninjured age-matched control larvae. Left and middle panels show whole zebrafish larvae at timepoints indicated, right panel shows tail fins of a representative experiment. Quantification shows number of larvae which look like representative image from 2 independent experiments.

Fig. 2 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. S1

CRISPR/Cas9 knockdown of tyrosinase does not affect neutrophil function A-B. Representative images of 2dpf mpx:GFP non-injected (A) and tyrosinase (B) mosaic pigment phenotypes. C. Whole body neutrophil counts in non-injected, vehicle control tracrRNA + cas9 protein injected and tyrosinase crRNA injected larvae. D. Neutrophils recruited to the injury site at 6hpi in 2dpf non-injected, vehicle control tracrRNA + cas9 protein injected and tyrosinase crRNA injected larvae. (Error bars shown are mean ± SEM. Groups were analysed using an ordinary one-way ANOVA and adjusted using Tukeys multi comparison test, n=30 from 3 independent repeats).

Acknowledgments:
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol