Sancataldo et al., 2019 - Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts. Frontiers in Neuroanatomy   13:7 Full text @ Front. Neuroanat.

Fig. 3

LSFM images of a zebrafish-larva brain taken with either a single or seven static light-sheets or with a single pivoted one. (B) Maps of spatial correlation along the x-axis (integrated over the y-axis), for the respective illumination configurations, with respect to the modeled stripe artifact. The images were generated by color-mapping the Pearson correlation coefficient, as detailed by the color bar, and the results for the different temporal frames of the time-lapse acquisition are displayed along the horizontal dimension. A subset of stripe artifacts produces fluorescence variations over time that lead to intermittent features in the correlation maps, like the one marked by the arrow. A second arrow in (A) marks the position of the corresponding artifact in the original image. Size scale bars of 50 μm, time scale bar of 2 s.

Fig. 4

 HSV images of a fluorescent zebrafish larva brain, for a single static (A), seven static (B), and a dynamically swept (C) light-sheet illumination. The average fluorescence intensity is mapped on the value channel, while the saturation channel and the hue channel are a function of the Pearson correlation coefficient, as detailed by the color-bar. The temporal correlation was computed between the pixel visualized in blue inside the white circle and every other pixel in the dataset. Striping artifacts, like the ones indicated by arrows, can be mistaken for biological-like activation events in functional live-studies of neural activity. White scale bar of 50 μm.

ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.