Generation of zebrafish transgenic lines expressing wild type and mutant human TDP-43. (A) The transgene constructs consist of the human wild type (TDP-43^WT) or the G348C mutant TDP-43 (TDP-43^G348C) cDNA fused with 6 myc-tag under the control of the hsp70-like promoter (hsp70l). The constructs also contained eGFP driven by the cardiac myosin light chain (cmlc2) promoter. (B) Representative picture of the embryos used for all experiments. (C) Western blot showing expression of the human transgene in 2 days post-fertilization (dpf) embryos following a 1 h heat shock at 38.5°C using an anti-myc antibody.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Abnormal development of motor neuron axons in zebrafish expressing TDP-43^G348C. (A) Immunohistochemistry against znp-1 in 2 dpf whole-mount embryos. The 3-to-4 somites spanning the anus are shown with the axons of the motor neuron expanding down from the spinal cord. White arrow heads indicate abnormal premature branching of the main axonal branch and absence of secondary branching in transgenic embryos expressing TDP-43^G348C, compared to embryos expressing TDP-43^WT or not expressing any transgene. (B) Classification criteria used for the quantification of defects. (C) Quantification of axonal defects based on the classification found in (B). The total number of axons analyzed is indicated; The three axons spanning the anus were analyzed in at least 30 embryos over the course of three experiments. Chi-square analysis was performed between the different conditions, *p-value < 0.05.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.