de Pater et al., 2018 - Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis. Frontiers in cell and developmental biology   6:124 Full text @ Front Cell Dev Biol


(A) Overview of definitive hematopoietic sites in the developing embryo where HSPCs are born from hemogenic endothelial cells of the dorsal aorta (DA). HSPCs are amplified in the caudal hematopoietic tissue (CHT) and migrate to the kidney and thymus. (B) Endothelial-to-hematopoietic transition (EHT) event imaged in a Tg(fli:GFP) embryo between 32 and 40 hpf. (C) CHT region in Tg(flt:RFP)/Tg(CD41:GFP) embryo at 56 hpf indicating erythroid myeloid progenitors (EMP) in green and definitive HSPCs in yellow as they originate from the artery and retain RFP at this timepoint. (D) Sagittal section through an adult zebrafish where the head kidney is indicated with enlargement showing the hematopoietic cells in between the kidney tubules.


Graph indicating different methods used to study zebrafish hematopoiesis.

ZFIN wishes to thank the journal Frontiers in cell and developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Cell Dev Biol