FIGURE SUMMARY
Title

Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b

Authors
Nasr, N., Faucherre, A., Borsotto, M., Heurteaux, C., Mazella, J., Jopling, C., Moha Ou Maati, H.
Source
Full text @ Sci. Rep.
Fig. S2

In situ hybridization on wild type zebrafish larvae at 4 days post fertilization (4 dpf) using an antisense probe against kcnk2a (A) or kcnk2b (B).

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 4
Fig. S3

Kcnk2a and kcnk2b transfection efficiency. (A) Brightfield image of HEK-293T transfected with kcnk2a-PIRES2 GFP. (B) GFP fluorescence image of HEK-293T transfected with kcnk2a-PIRES2 GFP. (C) Merged image of A and B. (D) Brightfield image of HEK-293T transfected with kcnk2b- PIRES2 GFP. (E) GFP fluorescence image of HEK-293T transfected with kcnk2b- PIRES2 GFP. (F) Merged image of D and E. (G) Graph showing the overall transfection efficiency of HEK-293T with either kcnk2a-PIRES2 GFP or kcnk2a- PIRES2 GFP. 8x35mm dishes were plated with 3x105 HEK-293T cells and transfected with either kcnk2a-PIRES2 GFP (4 dishes) or kcnk2a-PIRES2 GFP (4 dishes). 5 non overlapping random wide field images were obtained and the mean transfection efficiency was calculated for each dish. The data in (G) is the average of the combined means. Statistics: Students t test, NS (not significant) p>0,05.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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