Fig. 2

Effects of 4 G PEMF on pigmentation in zebrafish. (A) Synchronized embryos (n = 20) were exposed to the PEMF at the indicated intensity and frequency. The effects on zebrafish pigmentation were observed under a stereomicroscope via inferior views of the embryos at 15 dpf. (B) The pigmentation area density in the treated embryos, indicated by a white outline, was normalized to that of the control embryos using the ImageJ software. * p < 0.05, compared to the control. The scale bar is 500 μm.

Fig. 3

Effects of 4G PEMF in zebrafish at 15 dpf. Representative images of Fontana-Masson-stained zebrafish (dark color indicates secreted melanin). (A,C) control, (B,D) EMF. (E) the pigmented area density in figure (A–D) was normalized to that of the control, using ImageJ sotware. ** p < 0.05, compared to the control. Original magnification: (A,B) ×40; (C,D) × 100 scale bar; 100 μm.

Acknowledgments
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