Awan et al., 2018 - FGF2 Induces Migration of Human Bone Marrow Stromal Cells by Increasing Core Fucosylations on N-Glycans of Integrins. Stem Cell Reports   11(2):325-333 Full text @ Stem Cell Reports

Fig. 3

Silencing FUT8 Affects Migration of MSC in Zebrafish and Mice

(A) Representative image of GFP-expressing human MSCs around the head of 1-dpf zebrafish. Insert shows high magnification, where protrusions from an individual cell are visible.

(B) MSCs distributed around the trunk region.

(C) MSCs distributed in the tail. Distribution of transplanted cells is highlighted by arrows.

(D) Quantification of cell distribution, where p < 0.05 as assessed using a paired Student's t test (N = 5 independent experiments performed with biological replicates).

(E) Seven days after bone fracture and intramuscular injection of cells, mice were analyzed for presence of tdTomato-expressing mouse MSCs. Control cells distribute abundantly in callus and bone marrow of the fractured femur.

(F) MSCs with shFUT8 only minimally reach the new callus and bone marrow on fracture site.

Error bars indicate standard error of the mean (SEM). Scale bars, 100 μm.

Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports