Adult hippocampal progenitor cells transplanted at blastula stage are observed at least 5 days post-transplantation.

A) Representative image of blastula containing transplanted cells. B) Zebrafish with transplanted cells at 1, 2, 3, 4 and 5 dpf. Green = GFP-expressing AHPCs. Scale bar = 250 μm.

A large percentage of transplanted cells retain neural progenitor phenotypes.

Larvae at 3 dpf with transplanted AHPCs were immunolabeled for Nestin (red) at 3 dpf. Arrows indicate cells selected for higher magnification. A) Cells located at CNS and superficial regions were positive for Nestin. B) Cells in the zebrafish tail were Nestin positive. C) Quantification of average percent of Nestin+ cells/ location per fish at 3 dpf. N = 6. Error bars represent standard error of the mean.

Transplanted cells in the CNS adopted a neuronal fate.

A significant proportion of superficially-located cells were also neuronal, as indicated by TuJ1 immunolabeling (red) at 3 dpf. Arrows indicate cells selected for higher magnification. A) TuJ1+ cells were in the brain and at a superficial region. B) TuJ1+ cells in the brain and TuJ1- cells in facial cartilage. C) Quantification of the percent of TuJ1+ cells/location for each larvae at 3 dpf. One-way ANOVA with Dunn’s multiple comparisons test. N = 5. Error bars indicate standard error of the mean.

Representative image of transplanted AHPCs in the yolk periderm of a 1 dpf embryo exhibiting non-neural, flattened morphology.

Total number of cells located in the brain, superficial, or other regions per fish at 1, 3 and 5 days post-transplantation.

Acknowledgments
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