- Title
-
Vomocytosis of live pathogens from macrophages is regulated by the atypical MAP kinase ERK5
- Authors
- Gilbert, A.S., Seoane, P.I., Sephton-Clark, P., Bojarczuk, A., Hotham, R., Giurisato, E., Sarhan, A.R., Hillen, A., Velde, G.V., Gray, N.S., Alessi, D.R., Cunningham, D.L., Tournier, C., Johnston, S.A., May, R.C.
- Source
- Full text @ Sci Adv
ERK5 inhibition enhances vomocytosis and reduces dissemination in a zebrafish model of cryptococcosis. (A) Infected zebrafish treated with XMD17-109 show a significant (P = 0.001, Fisher’s exact test) increase in vomocytosis in vivo over 24 hours. Bars represent the median and 5th/95th percentile. (B) Overall fungal burden at 3 days after infection is not significantly altered by ERK5 inhibition. Bars represent the median and 5th/95th percentile, with the boxed region representing the interquartile range. (C) Individual infections can be individually scored as nondisseminated (restricted to the bloodstream; top), disseminated to muscle/tissue blocks (middle), or disseminated to the central nervous system (bottom). CNS, central nervous system. (D) Infected fish treated with XMD17-109 show significantly (P < 0.05, Fisher’s exact test) reduced dissemination from the site of infection. Bars represent the median and 5th/95th percentile. |