ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Effects of odor or glutamate stimulation on TN activity.

(A) Fluorescent and bright-field images of transgenic zebrafish Tg(GnRH3::GFP). The TNs were identified by the expression of GFP (arrows) and were recorded by patch-clamp methods. Scale bar: 200 μm. (B) Whole-cell patch-clamp recordings of the TN in response to odor (methionine, 1.2 mM applied to the nostril) or glutamate (0.5 mM, applied to the olfactory bulb) stimulation. Note the increase in membrane potential of the TNs in response to odor or glutamate stimulation. In all cases (n = 6), the holding potential was set at -90 mV. (C) Whole-cell patch-clamp recordings of the TNs in response to odor (methionine, 1.2 mM applied to the nostril) or glutamate (0.5 mM, applied to the olfactory bulb) stimulation in zebrafish in which the glutamate receptors were blocked by CNQX or D-AP-7. In response to odor or glutamate stimulation, no obvious changes were detected. In all cases (n = 6), the holding potential was set at -90 mV. (D) Effects of odor stimulation (methionine, 1.2 mM applied to the nostril) on spontaneous firing of the TN neuron. Note the increase of the firing in response to odor stimulation. Sham stimulation (with distilled water) produced no effect on the firing rate of the TN neuron. The experiments were performed using different TN neurons (n = 6). (E) Histograms of the firing of the TNs (n = 6) in response to sham (distilled water) or odor stimulation (methionine, 1.2 mM; applied to the nostril). Note the increase of the firing in response to odor stimulation.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Effects of GnRH-mediated olfacto-visual sensory integration on animal sexuality.

(A) Lateral view of transgenic zebrafish (GnRH::3-GFP; anterior to the left) that shows the TNs and TN axons in the brain (revealed by the expression of the GFP). Scale bar, 50 μm. (B) A cartoon showing the projection of TN axons (red) and the projection of RGC axons (green) to the hypothalamus. (C) GnRH signaling in the eye. Upon entering the retina, the TN axons synapse with DA-IPCs and RGCs. Most of the RGCs project axons to the visual cortex, but some of the RGCs project axons to the hypothalamus. Abbreviations: F, forebrain; H, hypothalamus; M, midbrain; P, photo receptor cells; R, retina; DA-IPC, dopaminergic interplexiform cell; INL, inner nuclear layer; IPL, inner plexiform layer; OB, olfactory bulb; ON, optic nerve; OPL, outer plexiform layer; RGC, retinal ganglion cells; TN, terminalis neuron.