Lamprey IGFBP-3 has insulin-like growth factor-dependent action in vivo. (A) Representative images of 24 hpf embryos injected with mRNA encoding the indicated proteins. Wild-type (WT) embryos were used as controls. Scale bar = 0.5 mm. Body length (B) and somite number (C) of the abovementioned groups were quantified and shown. Values are represented as means ± SD (n = 3). The total number of embryos in each group is shown on the top of each column. Groups with different letters are significantly different from each other (P < 0.05).