FIGURE SUMMARY
Title

Alcohol exposure leads to unrecoverable cardiovascular defects along with edema and motor function changes in developing zebrafish larvae

Authors
Li, X., Gao, A., Wang, Y., Chen, M., Peng, J., Yan, H., Zhao, X., Feng, X., Chen, D.
Source
Full text @ Biol. Open
Fig. 1

Phenotypic analysis, expression of cmlc2 and heart rate analysis after early exposure of alcohol. (A) Morphological characteristics of the heart in alcohol-exposed larvae. (B) Ventral view of the expression of cmlc2 in the heart of alcohol-treated larvae with the head at the top at 30, 54 and 78hpf. (C-E) Heart-rate analysis in ethanol-treated larvae at 30 hpf (C), 54 hpf (D) and 78 hpf (E). Red dotted line, atrium; white dotted line, ventricle. Error bars represent mean±s.e.m.; **P<0.01, ***P<0.001 by one-way ANOVA; n=48 for each group.
Fig. 2

The effect of alcohol exposure on the blood vessels of zebrafish larvae at 30, 54, 78 and 102hpf. (A-D) Morphology of blood vessels in control group at 30, 54, 78 and 102hpf. (E-H) Morphology of blood vessels in 1% group at 30, 54, 78 and 102hpf. (I-L) Morphology of blood vessels in 2% group at 30, 54, 78 and 102hpf.
Fig. 3

Morphology, histopathological examination and vascular structure in ethanol-treated and untreated larvae. (A-C) Morphological characteristics of ethanol-treated and untreated larvae at 6dpf. (D-I) H&E staining of transverse sections at site of abdomen in untreated and ethanol-treated larvae. Boxed areas in D,F,H magnified in E,G,I. (J,L,N) Reconstructed images of vascular structure in untreated and ethanol-treated larvae by confocal imaging. (K,M,O) Tomography of vascular structure in control and alcohol-treated larvae by confocal imaging. Asterisk in N,O indicates destruction in dorsal aorta.
Fig. 4

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Fig. S1

The effect of alcohol exposure on the expressions of amhc, vmhc and cmlc in zebrafish larvae at 30hpf.
Fig. S2

The effect of alcohol exposure on the expressions of amhc, vmhc and cmlc in zebrafish larvae at 54hpf.
Fig. S3

The effect of alcohol exposure on the expressions of amhc, vmhc and cmlc in zebrafish larvae at 78hpf.
Fig. S4

Phenotypic analysis of zebrafish Tg(cmlc2:egfp) embryos after early exposure of alcohol. (A) Lateral view of morphological characteristics of alcohol exposed larvae. (B) Statistical analyses for the body length of the larvae in untreated and alcohol treated groups. (C) Statistical analyses for the head area of the larvae in untreated and alcohol treated groups. (D) Statistical analyses for the eye area of the larvae in untreated and alcohol treated groups.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open
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