FIGURE SUMMARY
Title

Early-life glucocorticoids programme behaviour and metabolism in adulthood in zebrafish

Authors
Wilson, K.S., Tucker, C.S., Al-Dujaili, E.A., Holmes, M.C., Hadoke, P.W., Kenyon, C.J., Denvir, M.A.
Source
Full text @ J. Endocrinol.
Fig. 1

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Fig. 2

Effects of embryonic treatment on gross morphology of larvae. Images of whole larvae at 12h post fertilisation (hpf) (A, B, C and D), 18hpf (E, F, G and H) and 120hpf (I, J, K and L) show the effects of dexamethasone (B, F and J), hypoxia (C, G and K) and GR morpholino knockdown (GR Mo (D,h and L)) compared with untreated controls (A, E and I). Hypoxic larvae show reduced tail length and larger yolk sac and craniofacial immaturity. By contrast, dexamethasone-treated larvae show advanced eye development, somite patterning and yolk sac puckering. The delay in maturation in GR Mo is less prominent. The swim bladder appears less inflated in larvae with hypoxia and GR Mo treatment. Histological images of H&E-stained sections at two levels of magnification show typical examples of control (M), dexamethasone- (N), hypoxia- (O) and GR Mo-treated larvae at 120hpf (P). Features highlighted are swim bladder (SB), gut (G), yolk sac (YS), eye (E), heart (H) and brain (B). Analyses of images from 20 larvae/group were carried out in consultation with a veterinary pathologist.

Fig. 3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 5
Fig. 4

Effect of embryonic treatment on adult body mass, blood glucose and hepatic pepck expression. The effects of embryonic pre-treatment with dexamethasone, hypoxia and GR Mo on body length (A), mass (B) and condition (C) are shown for adult fish at 84dpf. Representative images of adult control (D), dexamethasone (E), hypoxic (F) and GR Mo pre-treated (G) fish show no signs of gross abnormality. Effects of embryonic treatment on adult blood glucose (H) and hepatic pepck mRNA expression (I). All data are mean±s.e.m. compared against respective controls by Student’s t-test (*P ≤ 0.05, ***P ≤ 0.001). Data for A, B and C are n=10 fish, data forh are n=8 fish (mean of 2 readings per fish) and n=3 (5 pooled livers per sample) for mRNA expression.

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Days 45-89
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 45-89
Fig. S1

Phenotype and mortality assessment.
text only

Unillustrated author statements

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Endocrinol.
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