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O'Connor et al., 2016 - Identification of mutations in the MYO9A gene in patients with congenital myasthenic syndrome. Brain : a journal of neurology   139(Pt 8):2143-53 Full text @ Brain

Fig. 4

Knockdown of MYO9A in zebrafish. (A) RT-PCR confirming knockdown success of myo9aa and myo9ab in zebrafish injected with morpholino oligonucleotide. There is loss of the myo9aa transcript and an alternatively spliced myo9ab transcript produced due to intron retention. Bands from the same gel have been cropped and put together for comparison, demarcated by the white lines. (B) Images of non-injected and mild/moderate myo9aa/ab morpholino oligonucleotide-injected zebrafish (48 hpf), with the latter displaying varying severities of tail curvature. Scale bar = 100 µm.

Fig. 5

Motor axons of morphant zebrafish are shortened and abnormally branched. Neuromuscular junctions in absence of Myo9aa/ab. Non-injected and myo9aa/ab morpholino oligonucleotide-injected embryos were stained for presynaptic motor axons (SV2, green) and postsynaptic AChRs (α-bungarotoxin, red) at 48 hpf. Morphant zebrafish display shortened (arrow) and abnormally branched motor axons, with some innervating adjacent myotomes (arrowhead). Scale bar = 100 µm.

Fig. S2 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-5 to Day 4

Fig. S4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. SVideo ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. SVideo10 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments:
ZFIN wishes to thank the journal Brain : a journal of neurology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Brain