Wan et al., 2016 - Transcriptome analysis reveals a ribosome constituents disorder involved in the RPL5 downregulated zebrafish model of Diamond-Blackfan anemia. BMC Medical Genomics   9:13 Full text @ BMC Med. Genomics

Fig. 1

Hemoglobin staining of embryos injected with RPL5 MO and the effectiveness of RPL5 MO. a-b Embryos co-injected with 25 ng RPL5:egfp DNA and 0.25 ng control MO produced green fluorescent protein (a), and the expression of the green fluorescent fusion protein was inhibited by co-injection with 0.25 ng RPL5 MO (b). c-e The different phenotype and its ratio of embryos injected with 0.25 ng RPL5 MO f-k The O-staining results revealed a drastic reduction in the number of hemoglobin-stained blood cells when RPL5 was knocked down (f and i show the control; g and j show the RPL5 knockdown), and this was partially rescued by coinjection with P53 MO (h and k). a, b, c, d, e,i, j and k show the lateral view; f, g, and h show the ventral view

Fig. 2

RPL5 is required for both primitive hematopoiesis and definitive hematopoiesis, which are partially mediated the by P53 pathway in zebrafish. a-c The expression of scl was significantly decreased in RPL5 MO zebrafish and was partly rescued in RPL5 and P53 double MO zebrafish at 12 hpf. d-i The expression of gata1 and pu1 was significantly decreased in RPL5 MO zebrafish and was partly rescued in RPL5 and P53 double MO zebrafish at 12 hpf. j-o The expression of c-myb and runx1 was significantly decreased in RPL5 MO zebrafish and was partly rescued in RPL5 and P53 double MO zebrafish at 48 hpf

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Med. Genomics