- Title
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Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse
- Authors
- Merrick Schill, E., Lake, J.I., Tusheva, O.A., Nagy, N., Bery, S.K., Foster, L., Avetisyan, M., Johnson, S.L., Stenson, W.F., Goldstein, A.M., Heuckeroth, R.O.
- Source
- Full text @ Dev. Biol.
Ibuprofen reduced ENCDC colonization of zebrafish bowel. (A–D) Zebrafish were treated with vehicle (1% DMSO) or ibuprofen (Ibu) from 34 to 96 hpf and then stained with HuC/D antibody. (A) White arrows indicate dorsal root ganglia. (C) White arrows highlight enteric neurons. Scale bar=500 µm. (B and D) Higher magnification of fish midsection. Scale bar=100 µm. (E) Length of bowel from most distal HuC/D+ cell (arrows in B and D) to bowel terminus (arrowheads in B and D). *P<0.05 (ANOVA on Ranks) (F) Length of whole zebrafish (1% DMSO n=4, 25 µM Ibuprofen n=6). *P=0.025 (t-test). (G) Distance between DRG and dorsal zebrafish edge (1% DMSO n=3, 25 µM ibuprofen n=4). *P>0.05 (t-test). |
Reprinted from Developmental Biology, 409(2), Merrick Schill, E., Lake, J.I., Tusheva, O.A., Nagy, N., Bery, S.K., Foster, L., Avetisyan, M., Johnson, S.L., Stenson, W.F., Goldstein, A.M., Heuckeroth, R.O., Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse, 473-88, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.